School of Life Sciences, Changchun Normal University, Changchun 130033, China.
School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Int J Mol Sci. 2024 May 30;25(11):5987. doi: 10.3390/ijms25115987.
Carbon dioxide (CO) released by plants can serve as a cue for regulating insect behaviors. is a widely distributed forestry pest that may use CO as a cue for foraging and oviposition. However, the molecular mechanism underlying its ability to sense CO has not been elucidated. Our initial study showed that CO is significantly attractive to adults. Subsequently, 44 gustatory receptors () were identified using transcriptome data, and 3 candidate CO receptors that are specifically expressed in the labial palps were identified. In vivo electrophysiological assays revealed that the labial palp is the primary organ for CO perception in , which is similar to findings in other lepidopteran species. By using the oocyte expression system, we showed that the and co-expressions produced a robust response to CO, but had an inhibitory effect on CO perception. Finally, immunohistochemical staining revealed sexual dimorphism in the CO-sensitive labial pit organ glomerulus (LPOG). Taken together, our results clarified the mechanism by which sense CO, laying the foundation for further investigations into the role of CO in the rapid spread of .
植物释放的二氧化碳（CO）可以作为调节昆虫行为的线索。舞毒蛾是一种广泛分布的林业害虫，它可能利用 CO 作为觅食和产卵的线索。然而，其感知 CO 的分子机制尚未阐明。我们的初步研究表明，CO 对舞毒蛾成虫具有显著的吸引力。随后，我们使用转录组数据鉴定了 44 个味觉受体（），并鉴定了 3 个在唇瓣中特异性表达的候选 CO 受体。体内电生理测定表明，唇瓣是舞毒蛾感知 CO 的主要器官，这与其他鳞翅目昆虫的研究结果相似。通过使用 卵母细胞表达系统，我们表明 和 共表达对 CO 产生了强烈的反应，但 对 CO 感知有抑制作用。最后，免疫组织化学染色显示，CO 敏感的唇瓣窝小球体（LPOG）存在性别二态性。综上所述，我们的研究结果阐明了舞毒蛾感知 CO 的机制，为进一步研究 CO 在其快速传播中的作用奠定了基础。
