School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China.
Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China.
J Agric Food Chem. 2024 Aug 21;72(33):18353-18364. doi: 10.1021/acs.jafc.4c02438. Epub 2024 Aug 6.
(Lepidoptera: Erebidae) is difficult and costly to control as a quarantine pest found globally. Sex pheromone trapping is an effective measure for its population monitoring and control; however, the peripheral neural mechanism of sex pheromone recognition in remains unclear. An electrophysiological analysis showed that both male and female moths of responded to four components of sex pheromones and the responses of male moths were stronger than those of the female moths. We identified three types of trichoid sensilla (ST) responsive to sex pheromones using the single sensillum recording technique. Each type was involved in recognizing 9, 10-epoxy-1, 3, 6-heneicosatriene (1, 3, 6-9, 10-epoxy-21Hy). Four peripheral neurons involved in the olfactory encoding of sex pheromones were identified. Four candidate pheromone receptor (PR) genes, , , , and , were screened by transcriptome sequencing. All of them were highly expressed in the antennae of males, except for , which was highly expressed in the antennae of females. Functional identification showed that HcunPR1a responded to sex pheromone. Other HcunPRs were not functionally identified. In summary, neurons involved in sex pheromone recognition of were located in the ST, and HcunPR1a recognized secondary pheromone components 1, 3, 6-9, 10epoxy-21Hy. Interestingly, PRs that recognize the main components of the sex pheromone may be located in an unknown branch of the olfactory receptor and merit further study. Our findings provide a better understanding of the peripheral neural coding mechanism of type II sex pheromones, and HcunPR1a may provide a target for the subsequent development of highly effective and specific biopesticides for .
（鳞翅目：夜蛾科）是一种全球性的检疫害虫，难以控制且防治成本高。性信息素诱捕是其种群监测和控制的有效措施；然而， 的性信息素识别的外围神经机制尚不清楚。电生理分析表明， 和 雌雄蛾均对性信息素的四个成分有反应，且雄蛾的反应强于雌蛾。我们使用单感器记录技术鉴定了三种对性信息素有反应的毛形感器（ST）。每一种类型都参与识别 9,10-环氧-1,3,6-heneicosatriene（1,3,6-9,10-环氧-21Hy）。鉴定出了四个参与性信息素嗅觉编码的外周神经元。通过转录组测序筛选出四个候选性信息素受体（PR）基因， ， ， ， 和 。除 外，它们在雄触角中均高度表达，而 则在雌触角中高度表达。功能鉴定表明 HcunPR1a 对性信息素有反应。其他 HcunPR 未被功能鉴定。综上所述， 性信息素识别涉及的神经元位于 ST 中，HcunPR1a 识别二级性信息素成分 1,3,6-9,10-环氧-21Hy。有趣的是，识别性信息素主要成分的 PR 可能位于嗅觉受体的未知分支中，值得进一步研究。我们的研究结果为 II 型性信息素的外围神经编码机制提供了更好的理解，HcunPR1a 可能为后续开发针对 的高效、特异性生物农药提供了一个靶标。
