Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 16500 Prague, Czech Republic.
Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 16500 Prague, Czech Republic.
Int J Mol Sci. 2024 May 30;25(11):6038. doi: 10.3390/ijms25116038.
Diarrhoea remains an important public health concern, particularly in developing countries, and has become difficult to treat because of antibacterial resistance. The development of synergistic antimicrobial agents appears to be a promising alternative treatment against diarrhoeic infections. In this study, the combined effect of tetracycline together with either nitroxoline, sanguinarine, or zinc pyrithione (representing various classes of plant-based compounds) was evaluated in vitro against selected diarrhoeic bacteria (, , , , , and ). The chequerboard method in 96-well microtiter plates was used to determine the sum of the fractional inhibitory concentration indices (FICIs). Three independent experiments were performed per combination, each in triplicate. It was observed that the combination of tetracycline with either nitroxoline, sanguinarine, or zinc pyrithione produced synergistic effects against most of the pathogenic bacteria tested, with FICI values ranging from 0.086 to 0.5. Tetracycline-nitroxoline combinations produced the greatest synergistic action against at a FICI value of 0.086. The combinations of the agents tested in this study can thus be used for the development of new anti-diarrhoeic medications. However, studies focusing on their in vivo anti-diarrhoeic activity and safety are required before any consideration for utilization in human medicine.
腹泻仍是一个重要的公共卫生关注点，特别是在发展中国家，由于抗菌药物耐药性，腹泻的治疗变得更加困难。协同抗菌药物的开发似乎是一种有前途的替代治疗腹泻感染的方法。在这项研究中，评估了四环素与硝呋太尔、血根碱或吡啶硫酮（代表不同类别的植物化合物）联合使用对选定的腹泻细菌（、、、、、）的体外协同作用。棋盘微量稀释法在 96 孔微量滴定板中用于确定部分抑菌浓度指数（FICI）的总和。每种组合进行了三次独立实验，每个实验重复三次。结果表明，四环素与硝呋太尔、血根碱或吡啶硫酮联合使用对大多数测试的致病菌产生协同作用，FICI 值范围为 0.086 至 0.5。四环素-硝呋太尔组合对产生协同作用最强，FICI 值为 0.086。因此，本研究中测试的药物组合可用于开发新的抗腹泻药物。然而，在考虑将其用于人类医学之前，需要进行关注其体内抗腹泻活性和安全性的研究。
