Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 21 Praha 6, Suchdol, Czech Republic.
Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, 323 00, Plzen, Czech Republic.
J Ethnopharmacol. 2021 Jan 10;264:113220. doi: 10.1016/j.jep.2020.113220. Epub 2020 Aug 15.
The prevalence of different types of chronic wounds, due to the ageing population and increase incidence of diseases, is becoming a worldwide problem. Various medicinal plants used in folk medicine have demonstrated wound healing and antimicrobial properties, and some of these species are currently used in commercial preparations. Despite the well-documented and rich tradition of the use of local herbs for the treatment of skin injuries in Samoan folk medicine, their wound healing potential has not yet been systematically studied.
Investigation into the in vitro antibacterial activity of ethanol extracts from 14 medicinal plants used in Samoan traditional medicine for the healing of wounds, burns and sores, and their effects on the proliferation and migration of human fibroblasts.
The antibacterial activity of these extracts was tested against pathogens associated with infected skin injuries, using the broth microdilution method. The effect on migration, proliferation and viability of human dermal fibroblasts was evaluated using wound healing scratch assay, cell proliferation assay, and thiazolyl blue tetrazolium bromide cytotoxicity test.
The extracts from Cerbera manghas, Commelina diffusa, Kleinhovia hospita, Mikania micrantha, Omalanthus nutans, Peperomia pellucida, Phymatosorus scolopendria, Piper graeffei, Psychotria insularum, and Schizostachyum glaucifolium inhibited the growth of Staphylococcus aureus at the minimum inhibitory concentration (MIC) of ≥4 μg/mL, whereas C. manghas and P. pellucida produced the same MIC against both Escherichia coli and Pseudomonas aeruginosa. Among the antibacterially active species, C. diffusa, K. hospita, P. scolopendria, P. insularum, and S. glaucifolium did not produce toxicity towards the standard line of normal adult human dermal fibroblasts (IC > 128 μg/mL). In addition, extracts from Barringtonia asiatica, C. manghas, M. micrantha, O. nutans, P. insularum, and Piper graeffei stimulated significant migration of dermal fibroblasts, while M. micrantha, O. nutans, and P. insularum did not affect cell proliferation at a concentration of 32 μg/mL.
The results suggest that the above-mentioned species of Samoan medicinal plants can be used for the development of new wound healing agents. However, further phytochemical and pharmacological research is needed regarding the isolation and identification of their active constituents.
由于人口老龄化和疾病发病率的增加，不同类型的慢性伤口的患病率正成为一个全球性问题。民间医学中使用的各种药用植物已被证明具有伤口愈合和抗菌特性，其中一些物种目前已用于商业制剂。尽管萨摩亚民间医学中有大量关于当地草药治疗皮肤损伤的有记载的丰富传统，但它们的伤口愈合潜力尚未得到系统研究。
研究萨摩亚传统医学中用于治疗伤口、烧伤和溃疡的 14 种药用植物的乙醇提取物的体外抗菌活性及其对人成纤维细胞增殖和迁移的影响。
使用肉汤微量稀释法测试这些提取物对与感染性皮肤损伤相关的病原体的抗菌活性。使用划痕愈合试验、细胞增殖试验和噻唑蓝溴化四唑细胞毒性试验评估对人真皮成纤维细胞迁移、增殖和活力的影响。
Cerbera manghas、Commelina diffusa、Kleinhovia hospita、Mikania micrantha、Omalanthus nutans、Peperomia pellucida、Phymatosorus scolopendria、Piper graeffei、Psychotria insularum 和 Schizostachyum glaucifolium 的提取物在最低抑菌浓度（MIC）≥4μg/mL 时抑制金黄色葡萄球菌的生长，而 C. manghas 和 P. pellucida 对大肠杆菌和铜绿假单胞菌产生相同的 MIC。在具有抗菌活性的物种中，C. diffusa、K. hospita、P. scolopendria、P. insularum 和 S. glaucifolium 对标准成人皮肤成纤维细胞（IC>128μg/mL）没有产生毒性。此外，来自 Barringtonia asiatica、C. manghas、M. micrantha、O. nutans、P. insularum 和 Piper graeffei 的提取物显著刺激真皮成纤维细胞的迁移，而 M. micrantha、O. nutans 和 P. insularum 在 32μg/mL 浓度下不影响细胞增殖。
结果表明，萨摩亚药用植物上述物种可用于开发新的伤口愈合剂。然而，需要进行进一步的植物化学和药理学研究，以分离和鉴定其活性成分。
