Institute of Medical Sciences, Tzu Chi University, Hualien 970374, Taiwan.
School of Medicine, Tzu Chi University, Hualien 970374, Taiwan.
Int J Mol Sci. 2024 May 30;25(11):6041. doi: 10.3390/ijms25116041.
Synthetic deer antler peptides (TSKYR, TSK, and YR) stimulate the proliferation of human chondrocytes and osteoblasts and increase the chondrocyte content of collagen and glycosamino-glycan in vitro. This study investigated the peptide mixture's pain relief and chondroprotective effect in a rat model of collagenase-induced osteoarthritis. Thirty-six adult male Sprague-Dawley rats were divided into three groups: control (saline), positive control (hyaluronic acid), and ex-perimental (peptides). Intra-articular collagenase injections were administered on days 1 and 4 to induce osteoarthritis in the left knees of the rats. Two injections of saline, hyaluronic acid, or the peptides were injected into the same knees of each corresponding group at the beginning of week one and two, respectively. Joint swelling, arthritic pain, and histopathological changes were evaluated. Injection of the peptides significantly reduced arthritic pain compared to the control group, as evidenced by the closer-to-normal weight-bearing and paw withdrawal threshold test results. Histological analyses showed reduced cartilage matrix loss and improved total cartilage degeneration score in the experimental versus the control group. Our findings suggest that intra-articular injection of synthetic deer antler peptides is a promising treatment for osteoarthritis.
合成鹿茸肽（TSKYR、TSK 和 YR）能刺激人软骨细胞和成骨细胞增殖，并增加体外培养的软骨细胞中胶原蛋白和糖胺聚糖的含量。本研究在胶原酶诱导的骨关节炎大鼠模型中，考察了肽混合物的镇痛和软骨保护作用。36 只成年雄性 Sprague-Dawley 大鼠分为三组：对照组（生理盐水）、阳性对照组（透明质酸）和实验组（肽）。在第 1 天和第 4 天，给大鼠的左膝关节内注射胶原酶，以诱导骨关节炎。在第 1 周和第 2 周开始时，分别向每组的同一膝关节内注射两次生理盐水、透明质酸或肽。评估关节肿胀、关节炎疼痛和组织病理学变化。与对照组相比，肽注射显著减轻了关节炎疼痛，这从更接近正常的负重和足底撤回阈值测试结果中可以看出。组织学分析显示，实验组的软骨基质丢失减少，总软骨退变评分改善。我们的研究结果表明，关节内注射合成鹿茸肽是治疗骨关节炎的一种有前途的方法。
