Ito Nanako, Yamasaki Sachiko, Shintani Tomoaki, Matsui Kensaku, Obayashi Fumitaka, Koizumi Koichi, Tani Ryouji, Yanamoto Souichi, Okamoto Tetsuji
Department of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima 734-8551, Japan.
Cancers (Basel). 2023 Apr 10;15(8):2221. doi: 10.3390/cancers15082221.
Tumor-infiltrating lymphocytes (TILs) have been used to predict the prognosis of solid tumors. In this study, we investigated which molecules in TILs play a role in the prognosis of patients with oral squamous cell carcinoma (OSCC).
In a retrospective case-control study, we immunohistochemically evaluated the expression of CD3, CD8, CD45RO, Granzyme B, and the major histocompatibility complex class I chain-related molecule A (MICA) of the histocompatibility complex as predictors of prognosis in 33 patients with OSCC. The patients were classified as TILs or TILs according to the number of TILs for each molecule in the central tumor (CT) and invasive margin (IM). Furthermore, MICA expression scores were determined based on the intensity of the staining.
CD45RO/TIL in the nonrecurrent group were significantly higher than those in the recurrent group in the CT and IM areas ( < 0.05). The disease-free survival/overall survival rate of the CD45RO/TILs group in the CT and IM areas and the Granzyme B/TILs group in the IM area was significantly lower than that of the CD45RO/TILs group and the Granzyme B/TILs group, respectively ( < 0.05). Furthermore, the MICA expression score of tumors around the CD45RO/TILs group was significantly higher than that of the CD45RO/TILs group ( < 0.05).
A high ratio of CD45RO-expressing TILs was associated with a disease-free/overall survival improvement in OSCC patients. Furthermore, the number of TILs that express CD45RO was associated with the expression of MICA in tumors. These results suggest that CD45RO-expressing TILs are useful biomarkers for OSCC.
肿瘤浸润淋巴细胞（TILs）已被用于预测实体瘤的预后。在本研究中，我们调查了TILs中的哪些分子在口腔鳞状细胞癌（OSCC）患者的预后中发挥作用。
在一项回顾性病例对照研究中，我们通过免疫组织化学评估了33例OSCC患者中CD3、CD8、CD45RO、颗粒酶B以及组织相容性复合体的主要组织相容性复合体I类链相关分子A（MICA）的表达，将其作为预后预测指标。根据中央肿瘤（CT）和浸润边缘（IM）中每个分子的TIL数量，将患者分为TILs或TILs。此外，根据染色强度确定MICA表达评分。
在CT和IM区域，非复发组的CD45RO/TIL显著高于复发组（<0.05）。CT和IM区域的CD45RO/TILs组以及IM区域的颗粒酶B/TILs组的无病生存/总生存率分别显著低于CD45RO/TILs组和颗粒酶B/TILs组（<0.05）。此外，CD45RO/TILs组周围肿瘤的MICA表达评分显著高于CD45RO/TILs组（<0.05）。
高比例表达CD45RO的TILs与OSCC患者无病/总生存改善相关。此外，表达CD45RO的TILs数量与肿瘤中MICA的表达相关。这些结果表明，表达CD4RO的TILs是OSCC的有用生物标志物。
