Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387 Cracow, Poland.
Bioimaging Laboratory, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdansk, Poland.
Int J Mol Sci. 2024 May 31;25(11):6089. doi: 10.3390/ijms25116089.
The genus (bladderworts) species are carnivorous plants that prey on invertebrates using traps with a high-speed suction mechanism. The outer trap surface is lined by dome-shaped glands responsible for secreting water in active traps. In terminal cells of these glands, the outer wall is differentiated into several layers, and even cell wall ingrowths are covered by new cell wall layers. Due to changes in the cell wall, these glands are excellent models for studying the specialization of cell walls (microdomains). The main aim of this study was to check if different cell wall layers have a different composition. Antibodies against arabinogalactan proteins (AGPs) were used, including JIM8, JIM13, JIM14, MAC207, and JIM4. The localization of the examined compounds was determined using immunohistochemistry techniques and immunogold labeling. Differences in composition were found between the primary cell wall and the cell secondary wall in terminal gland cells. The outermost layer of the cell wall of the terminal cell, which was cuticularized, was devoid of AGPs (JIM8, JIM14). In contrast, the secondary cell wall in terminal cells was rich in AGPs. AGPs localized with the JIM13, JIM8, and JIM14 epitopes occurred in wall ingrowths of pedestal cells. Our research supports the hypothesis of water secretion by the external glands.
（狸藻类）属物种是肉食性植物，它们使用具有高速抽吸机制的陷阱来捕食无脊椎动物。外陷阱表面由负责在活跃陷阱中分泌水的圆顶状腺体排列。在这些腺体的末端细胞中，外壁分化成数层，甚至细胞壁内突也被新的细胞壁层覆盖。由于细胞壁的变化，这些腺体是研究细胞壁特化（微区）的理想模型。本研究的主要目的是检查不同的细胞壁层是否具有不同的组成。使用了针对阿拉伯半乳聚糖蛋白 (AGPs) 的抗体，包括 JIM8、JIM13、JIM14、MAC207 和 JIM4。使用免疫组织化学技术和免疫金标记法确定了被检查化合物的定位。在终端腺细胞的初生细胞壁和次生细胞壁之间发现了组成上的差异。被角质化的末端细胞细胞壁的最外层不含 AGPs（JIM8、JIM14）。相比之下，末端细胞的次生细胞壁富含 AGPs。与 JIM13、JIM8 和 JIM14 表位结合的 AGPs 存在于基座细胞的细胞壁内突中。我们的研究支持了外部腺体通过分泌水来进行水分吸收的假说。
