• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

采用 qPCR 技术对 HLA-B75 亚型标志进行分子鉴定：卡马西平处方前更具包容性的药物遗传学方法。

Molecular identification of HLA-B75 serotype markers by qPCR: A more inclusive pharmacogenetic approach before carbamazepine prescription.

机构信息

Human Genetic Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.

Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

出版信息

Clin Transl Sci. 2024 Jun;17(6):e13867. doi: 10.1111/cts.13867.

DOI:10.1111/cts.13867
PMID:38894615
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11187875/
Abstract

Genetic screening for HLA-B15:02 before prescribing carbamazepine is standard practice to prevent severe cutaneous adverse reactions in Asian populations. These reactions are associated not only with this allele but also with closely related HLA-B75 serotype markers-HLA-B15:11 and HLA-B15:21-which are prevalent in several Asian countries. However, a reliable method for identifying HLA-B75 serotype markers is still not available. We developed an in-house quantitative PCR (qPCR) for HLA-B75 screening and validated it using 303 anonymized DNA samples. Due to inadequate quality control, the qPCR results for 11 samples were excluded. We analyzed the sensitivity and specificity of the test using 93 HLA-typed samples. The concordance between the qPCR method and an established screening method was assessed using 199 HLA-screened samples tested for HLA-B15:02 at Songklanagarind Hospital, Songkhla, Thailand. All discordant results were confirmed by Sanger sequencing. The qPCR method demonstrated a sensitivity of 100% (95% confidence interval = 83.16%-100.00%) and a specificity of 100% (95% confidence interval = 95.07%-100.00%). Concordance analysis revealed a 96.5% agreement between methods (192/199; 44 positive and 148 negative results). All discordant results were due to HLA-B75 markers not being HLA-B15:02 (two samples with HLA-B15:11 and five samples with HLA-B15:21). In conclusion, this qPCR method could be useful for identifying HLA-B75 carriers at risk of carbamazepine-induced reactions in Asian populations where carriers of HLA-B15:02, HLA-B15:11, or HLA-B15:21 are common.

摘要

在亚洲人群中，开处方卡马西平时进行 HLA-B15:02 基因筛查是标准做法，以预防严重的皮肤不良反应。这些反应不仅与该等位基因有关，还与密切相关的 HLA-B75 血清型标志物 HLA-B15:11 和 HLA-B15:21 有关，这些标志物在几个亚洲国家很常见。然而，目前仍然没有可靠的方法来识别 HLA-B75 血清型标志物。我们开发了一种内部定量 PCR（qPCR）用于 HLA-B75 筛查，并使用 303 份匿名 DNA 样本对其进行了验证。由于质量控制不足，有 11 个样本的 qPCR 结果被排除在外。我们使用 93 份 HLA 分型样本分析了该测试的灵敏度和特异性。使用在泰国 Songkhla 的 Songklanagarind 医院进行 HLA-B15:02 筛查的 199 份 HLA 筛查样本评估了 qPCR 方法与一种已建立的筛查方法之间的一致性。所有不一致的结果均通过 Sanger 测序进行了确认。qPCR 方法的灵敏度为 100%（95%置信区间=83.16%-100.00%），特异性为 100%（95%置信区间=95.07%-100.00%）。一致性分析显示两种方法之间有 96.5%的一致性（192/199；44 个阳性和 148 个阴性结果）。所有不一致的结果均归因于 HLA-B75 标志物不是 HLA-B15:02（两个样本为 HLA-B15:11，五个样本为 HLA-B15:21）。总之，这种 qPCR 方法可用于识别亚洲人群中卡马西平诱导反应风险的 HLA-B75 携带者，在这些人群中，HLA-B15:02、HLA-B15:11 或 HLA-B15:21 的携带者很常见。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/874fda4ea2b0/CTS-17-e13867-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/4d43ff6afcc8/CTS-17-e13867-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/101781256a7a/CTS-17-e13867-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/874fda4ea2b0/CTS-17-e13867-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/4d43ff6afcc8/CTS-17-e13867-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/101781256a7a/CTS-17-e13867-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/11187875/874fda4ea2b0/CTS-17-e13867-g002.jpg

相似文献

1
Molecular identification of HLA-B75 serotype markers by qPCR: A more inclusive pharmacogenetic approach before carbamazepine prescription.采用 qPCR 技术对 HLA-B75 亚型标志进行分子鉴定：卡马西平处方前更具包容性的药物遗传学方法。
Clin Transl Sci. 2024 Jun;17(6):e13867. doi: 10.1111/cts.13867.
2
Prescription of Controlled Substances: Benefits and Risks管制药品的处方：益处与风险
3
Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection.基于实验室的分子检测替代 RT-PCR 用于 SARS-CoV-2 感染的诊断。
Cochrane Database Syst Rev. 2024 Oct 14;10(10):CD015618. doi: 10.1002/14651858.CD015618.
4
Rapid, point-of-care antigen tests for diagnosis of SARS-CoV-2 infection.用于 SARS-CoV-2 感染诊断的快速、即时抗原检测。
Cochrane Database Syst Rev. 2022 Jul 22;7(7):CD013705. doi: 10.1002/14651858.CD013705.pub3.
5
Lamotrigine versus carbamazepine monotherapy for epilepsy: an individual participant data review.拉莫三嗪与卡马西平单药治疗癫痫的疗效比较：个体参与者数据回顾
Cochrane Database Syst Rev. 2018 Jun 28;6(6):CD001031. doi: 10.1002/14651858.CD001031.pub4.
6
Carbamazepine versus phenytoin monotherapy for epilepsy: an individual participant data review.卡马西平与苯妥英钠单药治疗癫痫：个体参与者数据回顾
Cochrane Database Syst Rev. 2017 Feb 27;2(2):CD001911. doi: 10.1002/14651858.CD001911.pub3.
7
The effect of sample site and collection procedure on identification of SARS-CoV-2 infection.样本采集部位和采集程序对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染鉴定的影响。
Cochrane Database Syst Rev. 2024 Dec 16;12(12):CD014780. doi: 10.1002/14651858.CD014780.
8
Carbamazepine versus phenytoin monotherapy for epilepsy: an individual participant data review.卡马西平与苯妥英单药治疗癫痫：个体参与者数据回顾
Cochrane Database Syst Rev. 2015 Aug 14(8):CD001911. doi: 10.1002/14651858.CD001911.pub2.
9
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.慢性斑块状银屑病的全身药理学治疗：一项网状Meta分析。
Cochrane Database Syst Rev. 2020 Jan 9;1(1):CD011535. doi: 10.1002/14651858.CD011535.pub3.
10
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.慢性斑块状银屑病的全身药理学治疗：一项网状荟萃分析。
Cochrane Database Syst Rev. 2017 Dec 22;12(12):CD011535. doi: 10.1002/14651858.CD011535.pub2.

本文引用的文献

1
South Asian maternal and paternal lineages in southern Thailand and the role of sex-biased admixture.泰国南部的南亚母系和父系血统以及性别偏倚混合的作用。
PLoS One. 2023 Sep 14;18(9):e0291547. doi: 10.1371/journal.pone.0291547. eCollection 2023.
2
Characterization of Drug-Specific CD4 T-Cells Reveals Possible Roles of HLA Class II in the Pathogenesis of Carbamazepine Hypersensitivity Reactions.药物特异性 CD4 T 细胞的特征分析揭示了 HLA II 类分子在卡马西平过敏反应发病机制中的可能作用。
Chem Res Toxicol. 2023 May 15;36(5):757-768. doi: 10.1021/acs.chemrestox.2c00414. Epub 2023 Apr 19.
3
Pharmacogenomics in Asians: Differences and similarities with other human populations.
亚洲人的药物基因组学：与其他人群的差异和相似性。
Expert Opin Drug Metab Toxicol. 2023 Jan;19(1):27-41. doi: 10.1080/17425255.2023.2178895. Epub 2023 Feb 18.
4
Development of label-free electrochemical impedance spectroscopy for the detection of HLA-B*15:02 and HLA-B*15:21 for the prevention of carbamazepine-induced Stevens-Johnson syndrome.用于检测 HLA-B*15:02 和 HLA-B*15:21 以预防卡马西平诱导的史蒂文斯-约翰逊综合征的无标记电化学阻抗谱的开发。
Anal Biochem. 2022 Dec 1;658:114931. doi: 10.1016/j.ab.2022.114931. Epub 2022 Oct 1.
5
HLA Allele-Restricted Immune-Mediated Adverse Drug Reactions: Framework for Genetic Prediction.HLA 等位基因限制的免疫介导的药物不良反应：遗传预测框架。
Annu Rev Pharmacol Toxicol. 2022 Jan 6;62:509-529. doi: 10.1146/annurev-pharmtox-052120-014115. Epub 2021 Sep 13.
6
Genetic variants associated with severe cutaneous adverse drug reactions induced by carbamazepine.与卡马西平引起的严重皮肤不良反应相关的遗传变异。
Br J Clin Pharmacol. 2022 Feb;88(2):773-786. doi: 10.1111/bcp.15022. Epub 2021 Aug 28.
7
Spectrum of cutaneous adverse reactions to aromatic antiepileptic drugs and human leukocyte antigen genotypes in Thai patients and meta-analysis.泰国患者中芳香族抗癫痫药物皮肤不良反应谱及人类白细胞抗原基因型与荟萃分析。
Pharmacogenomics J. 2021 Dec;21(6):682-690. doi: 10.1038/s41397-021-00247-3. Epub 2021 Jun 26.
8
as a potential biomarker for carbamazepine-induced Stevens-Johnson syndrome/toxic epidermal necrolysis in Filipino patients.作为菲律宾患者卡马西平诱导的史蒂文斯-约翰逊综合征/中毒性表皮坏死松解症的潜在生物标志物。
Pharmacogenomics. 2021 Jun;22(8):465-472. doi: 10.2217/pgs-2020-0191. Epub 2021 Apr 29.
9
Diagnostic Test Criteria for HLA Genotyping to Prevent Drug Hypersensitivity Reactions: A Systematic Review of Actionable HLA Recommendations in CPIC and DPWG Guidelines.预防药物超敏反应的HLA基因分型诊断测试标准：对CPIC和DPWG指南中可操作的HLA建议的系统评价
Front Pharmacol. 2020 Sep 23;11:567048. doi: 10.3389/fphar.2020.567048. eCollection 2020.
10
Genetic Diversity of Class I and Class II Alleles in Thai Populations: Contribution to Genotype-Guided Therapeutics.泰国人群中I类和II类等位基因的遗传多样性：对基因型指导治疗的贡献。
Front Pharmacol. 2020 Feb 27;11:78. doi: 10.3389/fphar.2020.00078. eCollection 2020.