Gerami Pedram, Tandukar Bishal, Deivendran Delahny, Olivares Shantel, Chen Limin, Tang Jessica, Tan Tuyet, Sharma Harsh, Bandari Aravind K, Cruz-Pacheco Noel, Chang Darwin, Marty Annika, Olshen Adam, Murad Natalia Faraj, Song Jing, Lee Jungwha, Yeh Iwei, Hunter Shain A
Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Department of Dermatology, University of California, San Francisco, San Francisco, CA, USA.
bioRxiv. 2024 Jun 5:2024.06.04.597225. doi: 10.1101/2024.06.04.597225.
Tanning bed users have a significantly increased risk of melanoma, but it remains unclear how indoor tanning drives melanomagenesis. Tanning bed radiation is often thought of as a substitute for natural UV radiation despite differences in the maximum doses, UV content, body sites exposed, and patterns of melanoma that arise.
To better understand the epidemiologic trends and etiology of melanoma associated with tanning bed use, we described the patterns of melanoma in patients with quantifiable tanning bed usage and performed exome sequencing of 182 melanocytes from normal skin of a subset of these patients.
Tanning bed users were more likely than non-users to have melanoma on body sites with low cumulative levels of sun damage and were more likely to have multiple melanomas. The melanocytes in normal appearing skin from tanning bed users had higher mutation burdens, a higher proportion of melanocytes with pathogenic mutations, and distinct mutational signatures. These differences were most prominent over body sites that experience comparatively less exposure to natural sunlight.
We conclude that tanning bed radiation induces melanoma by increasing the mutation burden of melanocytes and by mutagenizing a broader field of melanocytes than are typically exposed to natural sunlight. The unique signatures of mutations in skin cells of tanning users may be attributable to the distinct spectra of radiation emitted from solariums.
使用晒黑床的人患黑色素瘤的风险显著增加，但室内晒黑如何驱动黑色素瘤发生仍不清楚。尽管在最大剂量、紫外线含量、暴露的身体部位以及出现的黑色素瘤模式方面存在差异，但晒黑床辐射常被视为天然紫外线辐射的替代品。
为了更好地了解与使用晒黑床相关的黑色素瘤的流行病学趋势和病因，我们描述了有可量化晒黑床使用情况的患者的黑色素瘤模式，并对这些患者子集正常皮肤中的182个黑色素细胞进行了外显子组测序。
与未使用晒黑床的人相比，使用晒黑床的人在阳光损伤累积水平较低的身体部位更易患黑色素瘤，且更易患多发性黑色素瘤。使用晒黑床的人外观正常皮肤中的黑色素细胞具有更高的突变负荷、更高比例的带有致病性突变的黑色素细胞以及独特的突变特征。这些差异在相对较少暴露于自然阳光的身体部位最为明显。
我们得出结论，晒黑床辐射通过增加黑色素细胞的突变负荷以及诱变比通常暴露于自然阳光的范围更广的黑色素细胞领域来诱发黑色素瘤。晒黑使用者皮肤细胞中独特的突变特征可能归因于日光浴室发出的独特辐射光谱。
