Nakamura M, Dhople A M
Microbios. 1985;42(168):97-102.
The intrinsic energy potential of the fastidious mycobacterium, Mycobacterium lepraemurium, essential for growth in axenic liquid medium was determined by ultraviolet irradiation of the inoculum. The growth potential in NC-5 medium of irradiated inocula was measured by an ultrasensitive method of adenosine triphosphate (ATP) using a luciferin-luciferase acceptor system. The results indicate that growth of bacilli ceased when more than one third of the ATP pool in 7 X 10(6) cells was lost. The residual level of ATP is roughly equivalent to that found in 10(5) non-irradiated cells.
通过对接种物进行紫外线照射，测定了对麻风分枝杆菌生长至关重要的苛求分枝杆菌在无菌液体培养基中生长所需的内在能量潜力。使用荧光素-荧光素酶受体系统，通过超灵敏的三磷酸腺苷（ATP）方法测量了辐照接种物在NC-5培养基中的生长潜力。结果表明，当7×10⁶个细胞中超过三分之一的ATP库丢失时，杆菌生长停止。ATP的残留水平大致相当于10⁵个未辐照细胞中的水平。
