State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.
Bull Exp Biol Med. 2024 Apr;176(6):776-780. doi: 10.1007/s10517-024-06107-x. Epub 2024 Jun 19.
We studied a needle-free jet injection delivery of an experimental mRNA vaccine encoding the receptor-binding domain of the SARS-CoV-2 S protein (mRNA-RBD). Immunization of BALB/c mice with mRNA-RBD by a needle-free jet injector induced high levels of antibodies with virus-neutralizing activity and a virus-specific T-cell response. The immune response was low in the group of mice that received intramuscular injection of mRNA-RBD. The effectiveness of this simple and safe method of mRNA delivering has been demonstrated. Thus, jet injection of mRNA vaccine can be a good alternative to lipid nanoparticles.
我们研究了一种无针喷射注射装置来递呈编码 SARS-CoV-2 S 蛋白受体结合域的实验性 mRNA 疫苗（mRNA-RBD）。用无针喷射注射装置给 BALB/c 小鼠免疫 mRNA-RBD 会诱导产生高水平的具有病毒中和活性的抗体和病毒特异性 T 细胞应答。接受肌内注射 mRNA-RBD 的小鼠组中的免疫应答较低。这种简单安全的 mRNA 递呈方法的有效性已经得到了证明。因此，mRNA 疫苗的喷射注射可以作为脂质纳米粒的良好替代品。
