Cui Yao, He Wei, Li Ying, Ge Xizhen
College of Biochemical Engineering, Beijing Union University, Beijing, 100023, China.
Appl Biochem Biotechnol. 2024 Dec;196(12):8669-8684. doi: 10.1007/s12010-024-04979-3. Epub 2024 Jun 19.
Berberine (BBR) is widely used as a botanical pesticide due to its broad-spectrum antibacterial and antifungal activities. However, BBR degradation pathway in soil microorganisms, which determines its impact on soil environment, remains poorly understood. Herein, a novel BBR-degrading bacterium Agrobacterium sp. V1 was isolated and characterized. Agrobacterium sp. V1 was able to utilize BBR as the sole carbon source for cell growth, and 50 μg/mL of BBR was completely degraded within 48 h. To reveal the possible BBR degradation pathway, whole genome sequencing of Agrobacterium sp. V1 was conducted, and proteins in Agrobacterium sp. V1 were aligned with enzymes involved in BBR biosynthesis in Rhizoma Coptidis. The results indicated that more than 60% of enzymes in BBR biosynthesis pathway had orthologs in Agrobacterium sp. V1. Combined with the primary mass spectra of BBR metabolites, a novel BBR degradation pathway in this bacterium was proposed. In summary, the proposed BBR degradation pathway offered new insights into the impact of BBR to the environment and also provided a reference for studying BBR metabolism in microorganisms.
黄连素（BBR）因其广谱抗菌和抗真菌活性而被广泛用作植物源农药。然而，土壤微生物中BBR的降解途径，决定了其对土壤环境的影响，目前仍知之甚少。在此，分离并鉴定了一种新型的BBR降解细菌——农杆菌属V1菌株。农杆菌属V1菌株能够利用BBR作为细胞生长的唯一碳源，50μg/mL的BBR在48小时内被完全降解。为了揭示可能的BBR降解途径，对农杆菌属V1菌株进行了全基因组测序，并将农杆菌属V1菌株中的蛋白质与黄连中参与BBR生物合成的酶进行了比对。结果表明，BBR生物合成途径中60%以上的酶在农杆菌属V1菌株中有直系同源物。结合BBR代谢产物的一级质谱，提出了该细菌中一种新的BBR降解途径。总之，所提出的BBR降解途径为了解BBR对环境的影响提供了新的见解，也为研究微生物中BBR的代谢提供了参考。
