State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
School of Ecology and Environmental Science, Yunnan University, Kunming, China.
Elife. 2024 Jun 19;13:RP95502. doi: 10.7554/eLife.95502.
Microbes strongly affect invasive plant growth. However, how phyllosphere and rhizosphere soil microbes distinctively affect seedling mortality and growth of invaders across ontogeny under varying soil nutrient levels remains unclear. In this study, we used the invader to evaluate these effects. We found that higher proportions of potential pathogens were detected in core microbial taxa in leaf litter than rhizosphere soil and thus leaf inoculation had more adverse effects on seed germination and seedling survival than soil inoculation. Microbial inoculation at different growth stages altered the microbial community and functions of seedlings, and earlier inoculation had a more adverse effect on seedling survival and growth. The soil nutrient level did not affect microbe-mediated seedling growth and the relative abundance of the microbial community and functions involved in seedling growth. The effects of some microbial genera on seedling survival are distinct from those on growth. Moreover, the seedling-killing effects of fungal strains isolated from dead seedlings by non-sterile leaf inoculation exhibited significant phylogenetic signals, by which strains of and generally caused high seedling mortality. Our study stresses the essential role of litter microbes in population establishment by regulating seedling density and growth.
微生物强烈影响入侵植物的生长。然而，在不同土壤养分水平下，叶际和根际土壤微生物如何通过不同方式影响入侵植物幼苗的死亡率和生长，仍然不清楚。本研究以入侵植物 为研究对象，结果表明，在核心微生物类群中，叶凋落物中的潜在病原菌比例高于根际土壤，因此与土壤接种相比，叶片接种对种子萌发和幼苗存活的负面影响更大。在不同的生长阶段进行微生物接种会改变幼苗的微生物群落和功能，早期接种对幼苗存活和生长的负面影响更大。土壤养分水平不影响微生物介导的幼苗生长以及与幼苗生长相关的微生物群落和功能的相对丰度。一些微生物属对幼苗存活的影响与对生长的影响不同。此外，通过非无菌叶片接种从死亡幼苗中分离的真菌菌株对幼苗的致死效应表现出显著的系统发育信号，其中 和 菌株通常导致高的幼苗死亡率。本研究强调了叶凋落物微生物在通过调节幼苗密度和生长来建立种群方面的重要作用。
