文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

人参皂苷 Rg1 通过神经元细胞衍生的细胞外囊泡递送 MYCBP2 调节脊髓损伤后的免疫微环境和神经恢复。

Ginsenoside Rg1 Regulates Immune Microenvironment and Neurological Recovery After Spinal Cord Injury Through MYCBP2 Delivery via Neuronal Cell-Derived Extracellular Vesicles.

机构信息

Department of orthopaedics, Centre for Leading Medicine and Advanced Technologies of IHM, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China.

National Center for Translational Medicine (Shanghai) SHU Branch, Shanghai University, Shanghai, 200444, China.

出版信息

Adv Sci (Weinh). 2024 Aug;11(31):e2402114. doi: 10.1002/advs.202402114. Epub 2024 Jun 19.

DOI:10.1002/advs.202402114
PMID:38896802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11336912/
Abstract

Spinal cord injury (SCI) is a severe neurological condition that frequently leads to significant sensory, motor, and autonomic dysfunction. This study sought to delineate the potential mechanistic underpinnings of extracellular vesicles (EVs) derived from ginsenoside Rg1-pretreated neuronal cells (Rg1-EVs) in ameliorating SCI. These results demonstrated that treatment with Rg1-EVs substantially improved motor function in spinal cord-injured mice. Rg1-EVs enhance microglial polarization toward the M2 phenotype and repressed oxidative stress, thereby altering immune responses and decreasing inflammatory cytokine secretion. Moreover, Rg1-EVs substantially diminish reactive oxygen species accumulation and enhanced neural tissue repair by regulating mitochondrial function. Proteomic profiling highlighted a significant enrichment of MYCBP2 in Rg1-EVs, and functional assays confirmed that MYCBP2 knockdown counteracted the beneficial effects of Rg1-EVs in vitro and in vivo. Mechanistically, MYCBP2 is implicated in the ubiquitination and degradation of S100A9, thereby promoting microglial M2-phenotype polarization and reducing oxidative stress. Overall, these findings substantiated the pivotal role of Rg1-EVs in neuronal protection and functional recovery following SCI through MYCBP2-mediated ubiquitination of S100A9. This research offers novel mechanistic insights into therapeutic strategies against SCI and supports the clinical potential of Rg1-EVs.

摘要

脊髓损伤（SCI）是一种严重的神经系统疾病，常导致明显的感觉、运动和自主功能障碍。本研究旨在阐述来源于人参皂苷 Rg1 预处理神经元细胞的细胞外囊泡（EVs）在改善 SCI 中的潜在机制基础。这些结果表明，Rg1-EVs 治疗可显著改善脊髓损伤小鼠的运动功能。Rg1-EVs 增强小胶质细胞向 M2 表型的极化并抑制氧化应激，从而改变免疫反应并减少炎症细胞因子的分泌。此外，Rg1-EVs 通过调节线粒体功能显著减少活性氧物质的积累并增强神经组织修复。蛋白质组学分析突出显示 Rg1-EVs 中 MYCBP2 的显著富集，功能测定证实 MYCBP2 敲低可拮抗 Rg1-EVs 在体外和体内的有益作用。在机制上，MYCBP2 参与 S100A9 的泛素化和降解，从而促进小胶质细胞 M2 表型极化并减少氧化应激。总的来说，这些发现证实了 Rg1-EVs 通过 MYCBP2 介导的 S100A9 泛素化在 SCI 后神经元保护和功能恢复中的关键作用。该研究为 SCI 的治疗策略提供了新的机制见解，并支持 Rg1-EVs 的临床潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/4d63a337f9d9/ADVS-11-2402114-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/dac46cdf0431/ADVS-11-2402114-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/54d6d97a69a3/ADVS-11-2402114-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/f9d212b77012/ADVS-11-2402114-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/e1e7c0b2c804/ADVS-11-2402114-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/7aa3b8c8f3c2/ADVS-11-2402114-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/ae8c962b766e/ADVS-11-2402114-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/fa95583eff23/ADVS-11-2402114-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/b522b64fbbca/ADVS-11-2402114-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/a7817ae31801/ADVS-11-2402114-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/fce0d9710f06/ADVS-11-2402114-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/4d63a337f9d9/ADVS-11-2402114-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/dac46cdf0431/ADVS-11-2402114-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/54d6d97a69a3/ADVS-11-2402114-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/f9d212b77012/ADVS-11-2402114-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/e1e7c0b2c804/ADVS-11-2402114-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/7aa3b8c8f3c2/ADVS-11-2402114-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/ae8c962b766e/ADVS-11-2402114-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/fa95583eff23/ADVS-11-2402114-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/b522b64fbbca/ADVS-11-2402114-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/a7817ae31801/ADVS-11-2402114-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/fce0d9710f06/ADVS-11-2402114-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfd/11336912/4d63a337f9d9/ADVS-11-2402114-g009.jpg

相似文献

[1]
Ginsenoside Rg1 Regulates Immune Microenvironment and Neurological Recovery After Spinal Cord Injury Through MYCBP2 Delivery via Neuronal Cell-Derived Extracellular Vesicles.

Adv Sci (Weinh). 2024-8

[2]
Ginsenoside Rg1 inhibits oxidative stress and inflammation in rats with spinal cord injury via Nrf2/HO-1 signaling pathway.

Neuroreport. 2022-1-19

[3]
Ginsenoside Rg1 regulates astrocytes to promote angiogenesis in spinal cord injury via the JAK2/STAT3 signaling pathway.

J Ethnopharmacol. 2024-11-15

[4]
Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury.

J Nanobiotechnology. 2021-9-8

[5]
Isorhamnetin promotes functional recovery in rats with spinal cord injury by abating oxidative stress and modulating M2 macrophages/microglia polarization.

Eur J Pharmacol. 2021-3-15

[6]
Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.

J Pineal Res. 2021-12

[7]
Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury.

Cell Commun Signal. 2024-2-29

[8]
Oral treatment with glycyrrhizin inhibits NLRP3 inflammasome activation and promotes microglial M2 polarization after traumatic spinal cord injury.

Brain Res Bull. 2020-5

[9]
Ginsenoside Rg1-induced activation of astrocytes promotes functional recovery via the PI3K/Akt signaling pathway following spinal cord injury.

Life Sci. 2020-4-4

[10]
MicroRNA-421-3p-abundant small extracellular vesicles derived from M2 bone marrow-derived macrophages attenuate apoptosis and promote motor function recovery via inhibition of mTOR in spinal cord injury.

J Nanobiotechnology. 2020-5-13

引用本文的文献

[1]
Microglial pyroptosis as a therapeutic target after traumatic spinal cord injury: current progress and future directions.

Front Immunol. 2025-8-22

[2]
Exosomes Derived from Tanshinone IIA-Pretreated Umbilical Cord Mesenchymal Stem Cells Repair Traumatic Spinal Cord Injury by miR-223-5p/USP8/NLRP3 Axis.

ACS Appl Mater Interfaces. 2025-8-13

[3]
Effect of microglial Pd1 on glial scar formation after spinal cord injury in mice.

J Biol Chem. 2025-5

[4]
Neurological Emergency Treatment Strategy: A Neuron-Targeted Regulation System for Reactive Oxygen Species Metabolism through Ferroptosis Modulation.

ACS Nano. 2025-3-11

本文引用的文献

[1]
Exosomes derived from CD271CD56 bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury.

Theranostics. 2024

[2]
Exosome-mediated repair of spinal cord injury: a promising therapeutic strategy.

Stem Cell Res Ther. 2024-1-2

[3]
Small Extracellular Vesicles Derived from Altered Peptide Ligand-Loaded Dendritic Cell Act as A Therapeutic Vaccine for Spinal Cord Injury Through Eliciting CD4 T cell-Mediated Neuroprotective Immunity.

Adv Sci (Weinh). 2024-1

[4]
Cytoplasmic Escape of Mitochondrial DNA Mediated by Mfn2 Downregulation Promotes Microglial Activation via cGas-Sting Axis in Spinal Cord Injury.

Adv Sci (Weinh). 2024-1

[5]
Hypoxic Preconditional Engineering Small Extracellular Vesicles Promoted Intervertebral Disc Regeneration by Activating Mir-7-5p/NF-Κb/Cxcl2 Axis.

Adv Sci (Weinh). 2023-12

[6]
Iridium metal complex targeting oxidation resistance 1 protein attenuates spinal cord injury by inhibiting oxidative stress-associated reactive oxygen species.

Redox Biol. 2023-11

[7]
Targeted Delivery of RGD-CD146CD271 Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood-Spinal Cord Barrier Repair after Spinal Cord Injury.

ACS Nano. 2023-9-26

[8]
Inflammation Modifies miR-21 Expression Within Neuronal Extracellular Vesicles to Regulate Remyelination Following Spinal Cord Injury.

Stem Cell Rev Rep. 2023-8

[9]
Ginsenoside Rg1 attenuates cerebral ischemia-reperfusion injury through inhibiting the inflammatory activation of microglia.

Exp Cell Res. 2023-5-1

[10]
SIRT1 attenuates blood-spinal cord barrier disruption after spinal cord injury by deacetylating p66Shc.

Redox Biol. 2023-4

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索