NIZO food research B.V., 6718 ZB Ede, the Netherlands.
FrieslandCampina, Stationsplein 4, 3818 LE, Amersfoort, the Netherlands.
Benef Microbes. 2024 Jun 20;15(4):373-385. doi: 10.1163/18762891-bja00017.
Sleep quality and duration can be impacted by diet, and has been linked to gut microbiota composition and function as the result of communication via the microbiota-gut-brain axis. As one strategy to improve sleep quality could be through the modulation of the gut microbiome, we assessed the effects of a dairy-based product containing whey protein, galacto-oligosaccharides, tryptophan, vitamins and minerals after a 3 weeks intervention on gut microbiota composition and (gut-brain related) functions on basis of 67 healthy subjects with moderate sleep disturbances. Associations of the gut microbiota with sleep quality and with response/non-response to the treatment were revealed by shotgun metagenomics sequencing of faecal DNA samples, and subsequent analyses of microbiota taxonomy and generic functionality. A database of manually curated Gut-Brain Modules (GBMs) was applied to analyse specific microbial functions/pathways that have the potential to interact with the brain. A moderate discriminating effect of the DP treatment on gut microbiota composition was revealed which could be mainly attributed to a decrease in Pseudomonas resinovorans, Flintibacter sp. KGM00164, Intestinimonas butyriciproducens, and Flavonifractor plautii. As interindividual variance in microbiota composition could have given rise to a heterogenous responsiveness of the subjects in the intervention group, we zoomed in on the differences between responders and non-responders. A significant difference in baseline microbiota composition between responders and non-responders was apparent, showing lower Bifidobacterium longum and Bifidobacterium adolescentis, and higher Faecalibacterium prausnitzii relative abundances in responders. The findings provide leads with respect to the effectiveness and potential underlying mechanisms of mode of action in sleep improvement that could support future nutritional interventions to aid sleep improvement.
睡眠质量和时长可能会受到饮食的影响，并且由于通过微生物群-肠道-大脑轴进行的通讯，已经与肠道微生物群落组成和功能相关联。由于通过调节肠道微生物群来改善睡眠质量可能是一种策略，因此我们评估了在 67 名有中度睡眠障碍的健康受试者中，经过 3 周的干预后，含有乳清蛋白、半乳糖寡糖、色氨酸、维生素和矿物质的乳制品对肠道微生物群落组成和（与肠道-大脑相关）功能的影响。通过对粪便 DNA 样本进行 shotgun 宏基因组测序，随后对微生物群落分类和通用功能进行分析，揭示了肠道微生物群落与睡眠质量的相关性，以及对治疗的反应/无反应的相关性。应用手动编辑的肠道-大脑模块 (GBMs) 数据库来分析具有与大脑相互作用潜力的特定微生物功能/途径。DP 治疗对肠道微生物群落组成有适度的区分作用，这主要归因于 Pseudomonas resinovorans、Flintibacter sp. KGM00164、Intestinimonas butyriciproducens 和 Flavonifractor plautii 的减少。由于个体间微生物群落组成的差异可能导致干预组中受试者的反应具有异质性，因此我们关注了反应者和无反应者之间的差异。在反应者和无反应者之间，基线微生物群落组成存在显著差异，表现为反应者中双歧杆菌长双歧杆菌和双歧杆菌青少年双歧杆菌的丰度较低，而粪肠球菌丰度较高。这些发现为改善睡眠的有效性和潜在作用机制提供了线索，这可能支持未来的营养干预措施来帮助改善睡眠。
