Laboratório de Produtos e Derivados Naturais, Laboratório de Investigação Médica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina de São Paulo, Universidade de São Paulo, São Paulo 01246-903, SP, Brazil.
Pesquisa e Desenvolvimento Efeom Nutrição S/A, São Paulo 03317-000, SP, Brazil.
Nutrients. 2024 Sep 10;16(18):3049. doi: 10.3390/nu16183049.
Stress, unhealthy lifestyle, and sleep disturbance worsen cognitive function in mood disorders, prompting a rise in the development of integrative health approaches. The recent investigations in the gut-brain axis field highlight the strong interplay among microbiota, inflammation, and mental health. Thus, this study aimed to investigate a new nutraceutical formulation comprising prebiotics, minerals, and silymarin's impact on microbiota, inflammation, mood, and sleep quality. The study evaluated the LL1 + silymarin capsule supplementation over 180 days in overweight adults. We analyzed the fecal gut microbiota using partial 16S rRNA sequences, measured cytokine expression via CBA, collected anthropometric data, quality of life, and sleep questionnaire responses, and obtained plasma samples for metabolic and hormonal analysis at baseline (T0) and 180 days (T180) post-supplementation. Our findings revealed significant reshaping in gut microbiota composition at the phylum, genus, and species levels, especially in the butyrate-producer bacteria post-supplementation. These changes in gut microbiota were linked to enhancements in sleep quality, mood perception, cytokine expression, and anthropometric measures which microbiota-derived short-chain fatty acids might enhance. The supplementation tested in this study seems to be able to improve microbiota composition, reflecting anthropometrics and inflammation, as well as sleep quality and mood improvement.
压力、不健康的生活方式和睡眠障碍会使情绪障碍患者的认知功能恶化，促使人们更多地采用综合健康方法。最近在肠道-大脑轴领域的研究强调了微生物群、炎症和心理健康之间的紧密相互作用。因此，本研究旨在探讨一种新的营养配方，该配方包含益生元、矿物质和水飞蓟素，以研究其对微生物群、炎症、情绪和睡眠质量的影响。该研究评估了超重成年人补充 LL1 + 水飞蓟素胶囊 180 天的效果。我们使用部分 16S rRNA 序列分析粪便肠道微生物群，通过 CBA 测量细胞因子表达，收集人体测量数据、生活质量和睡眠问卷应答，并在基线（T0）和补充后 180 天（T180）获得血浆样本进行代谢和激素分析。我们的研究结果表明，在门、属和种水平上，肠道微生物群的组成发生了显著变化，尤其是在补充后丁酸产生菌。这些肠道微生物群的变化与睡眠质量、情绪感知、细胞因子表达和人体测量指标的改善有关，而微生物衍生的短链脂肪酸可能会增强这些指标。本研究中测试的补充剂似乎能够改善微生物群的组成，反映出人体测量和炎症以及睡眠质量和情绪的改善。
