Looijesteijn Ellen, Schoemaker Marieke H, van den Belt Maartje, Hester Eric R, Kortman Guus A M, Viskaal-van Dongen Mirre, Nauta Arjen
FrieslandCampina, Amersfoort, Netherlands.
Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, Netherlands.
Front Nutr. 2024 Aug 19;11:1440319. doi: 10.3389/fnut.2024.1440319. eCollection 2024.
Galacto-oligosaccharides (GOS) are well-substantiated prebiotic substrates. Multiple studies have demonstrated a positive impact of GOS on gut microbiota composition and activity, so-far mainly related to . However, data on the beneficial impact at lower dosages in a healthy female population are limited. The primary aim of the current study was to reveal the effect of low dosages (1.3 and 2.0 g) of GOS on fecal abundance in healthy women. Other outcomes included the effect of low dosage of GOS on overall fecal microbiota composition and on self-perceived GI comfort, sleep quality and mental wellbeing.
Eighty-eight healthy women (42-70 years, BMI 18.7-30 kg/m) were included in this randomized, parallel, double-blind study of 6 weeks. The participants were stratified for fiber intake, BMI and age and randomized to consume either 1.3 or 2.0 g of GOS per day for 3 weeks after a control period of 3 weeks without any intervention. Fecal samples were collected for shotgun metagenomics sequencing at the start (t = -3) and end (t = 0) of the control period and at the end of the intervention period (t = 3). Self-perceived gut comfort, sleep quality, and mental wellbeing were assessed weekly. Hierarchical clustering of principal components was applied to data collected from study participants.
The relative abundance of in feces increased significantly after 3 weeks of daily consumption of both 1.3 g ( < 0.01) and 2.0 g GOS ( < 0.01). This was accompanied by a significant shift in the overall microbiota composition for the dosage of 2.0 g GOS ( < 0.01). Participants that showed a larger increase in in the intervention period compared to the change in in the control period, defined as responders, showed a significant overall difference in initial fecal microbiota composition as compared to non-responders ( = 0.04) and a trend towards lower baseline levels of in responders ( = 0.10).
Daily consumption of a low dose of GOS can lead to an increase in the relative abundance of in feces of healthy women. Additionally, with 2.0 g GOS, the enrichment of is accompanied with a shift in the overall microbiota composition.: clinicaltrials.gov, identifier NCT05762965.
低聚半乳糖（GOS）是有充分证据的益生元底物。多项研究已证明GOS对肠道微生物群组成和活性有积极影响，迄今为止主要涉及[此处原文缺失相关内容]。然而，关于较低剂量对健康女性群体有益影响的数据有限。本研究的主要目的是揭示低剂量（1.3克和2.0克）GOS对健康女性粪便中[此处原文缺失相关内容]丰度的影响。其他结果包括低剂量GOS对整体粪便微生物群组成以及自我感知的胃肠道舒适度、睡眠质量和心理健康的影响。
88名健康女性（42 - 70岁，BMI 18.7 - 30 kg/m）被纳入这项为期6周的随机、平行、双盲研究。参与者根据纤维摄入量、BMI和年龄进行分层，在为期3周的无任何干预的对照期后，随机分配每天食用1.3克或2.0克GOS，持续3周。在对照期开始（t = -3）和结束（t = 0）时以及干预期结束（t = 3）时采集粪便样本进行鸟枪法宏基因组测序。每周评估自我感知的肠道舒适度、睡眠质量和心理健康。对从研究参与者收集的数据应用主成分层次聚类。
每天食用1.3克（P < 0.01）和2.0克GOS（P < 0.01）3周后，粪便中[此处原文缺失相关内容]的相对丰度显著增加。对于2.0克GOS的剂量，这伴随着整体微生物群组成的显著变化（P < 0.01）。在干预期内与对照期相比[此处原文缺失相关内容]增加幅度更大的参与者（定义为反应者），与无反应者相比，初始粪便微生物群组成存在显著总体差异（P = 0.04），且反应者中[此处原文缺失相关内容]的基线水平有降低趋势（P = 0.10）。
每天食用低剂量GOS可导致健康女性粪便中[此处原文缺失相关内容]的相对丰度增加。此外，食用2.0克GOS时，[此处原文缺失相关内容]的富集伴随着整体微生物群组成的变化。：clinicaltrials.gov，标识符NCT05762965 。
