Department of Pathology, University of Helsinki, 00014, Helsinki, Finland.
Department of Pathology, University of Helsinki and Helsinki University Hospital, 00290, Helsinki, Finland.
Sci Rep. 2024 Jun 19;14(1):14148. doi: 10.1038/s41598-024-64823-w.
The increasing incidence of oropharyngeal squamous cell carcinoma (OPSCC) is primarily due to human papillomavirus, and understanding the tumor biology caused by the virus is crucial. Our goal was to investigate the proteins present in the serum of patients with OPSCC, which were not previously studied in OPSCC tissue. We examined the difference in expression of these proteins between HPV-positive and -negative tumors and their correlation with clinicopathological parameters and patient survival. The study included 157 formalin-fixed, paraffin-embedded tissue samples and clinicopathological data. Based on the protein levels in the sera of OPSCC patients, we selected 12 proteins and studied their expression in HPV-negative and HPV-positive OPSCC cell lines. LRG1, SDR16C5, PIP4K2C and MVD proteins were selected for immunohistochemical analysis in HPV-positive and -negative OPSCC tissue samples. These protein´s expression levels were compared with clinicopathological parameters and patient survival to investigate their clinical relevance. LRG1 expression was strong in HPV-negative whereas SDR16C5 expression was strong in HPV-positive tumors. Correlation was observed between LRG1, SDR16C5, and PIP4K2C expression and patient survival. High expression of PIP4K2C was found to be an independent prognostic factor for overall survival and expression correlated with HPV-positive tumor status. The data suggest the possible role of LRG1, SDR16C5 and PIP4K2C in OPSCC biology.
口咽鳞状细胞癌（OPSCC）的发病率不断上升，主要与人类乳头瘤病毒（HPV）有关，了解病毒引起的肿瘤生物学特性至关重要。我们的目标是研究 OPSCC 患者血清中以前未在 OPSCC 组织中研究过的蛋白质。我们研究了 HPV 阳性和阴性肿瘤之间这些蛋白质表达的差异及其与临床病理参数和患者生存的相关性。该研究纳入了 157 例福尔马林固定、石蜡包埋的组织样本和临床病理数据。基于 OPSCC 患者血清中的蛋白质水平，我们选择了 12 种蛋白质，并研究了它们在 HPV 阴性和 HPV 阳性 OPSCC 细胞系中的表达。选择 LRG1、SDR16C5、PIP4K2C 和 MVD 蛋白进行 HPV 阳性和阴性 OPSCC 组织样本的免疫组织化学分析。比较这些蛋白质的表达水平与临床病理参数和患者生存情况，以探讨其临床相关性。LRG1 在 HPV 阴性肿瘤中表达较强，而 SDR16C5 在 HPV 阳性肿瘤中表达较强。LRG1、SDR16C5 和 PIP4K2C 的表达与患者生存相关。发现 PIP4K2C 的高表达是总生存的独立预后因素，且其表达与 HPV 阳性肿瘤状态相关。数据提示 LRG1、SDR16C5 和 PIP4K2C 可能在 OPSCC 生物学中发挥作用。
