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使用光学显微镜和摄影测量法对珍珠进行三维微观重建。

3D microscopic reconstruction of pearls using combined optical microscopy and photogrammetry.

作者信息

Edeline Paul-Emmanuel, Leclercq Mickaël, Le Luyer Jérémy, Droit Arnaud, Chabrier Sébastien

机构信息

Département de médecine moléculaire, Faculté de Médecine, Université Laval, Québec, G1V 0A6, Canada.

Géopole du Pacifique Sud, Université de Polynésie Française, 98704, Faa'a, Tahiti, Polynésie Française, France.

出版信息

Sci Rep. 2024 Jun 20;14(1):14180. doi: 10.1038/s41598-024-64680-7.

DOI:10.1038/s41598-024-64680-7
PMID:38898152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11186833/
Abstract

In this study, we introduce an affordable and accessible method that combines optical microscopy and photogrammetry to reconstruct 3D models of Tahitian pearls. We present a novel device designed for acquiring microscopic images around a sphere using translational displacement stages and outline our method for reconstructing these images. We successfully created 3D models of two individual pearl rings, each representing 6.3% of the pearl's surface. Additionally, we generated a combined model representing 10.3% of the pearl's surface. This showcases the potential for reconstructing entire pearls with appropriate instrumentation. We emphasize that our approach extends beyond pearls and spherical objects and can be adapted for various object types using appropriate acquisition devices. We provide a proof of concept demonstrating the feasibility of 3D photogrammetry using optical microscopy. Consequently, our method offers a practical and cost-effective alternative for generating 3D models at a microscopic scale, particularly when detailed internal structure information is unnecessary.

摘要

在本研究中,我们介绍了一种经济实惠且易于使用的方法,该方法结合了光学显微镜和摄影测量技术来重建大溪地珍珠的三维模型。我们展示了一种新颖的设备,该设备通过平移位移台获取球体周围的微观图像,并概述了我们重建这些图像的方法。我们成功创建了两个单独珍珠环的三维模型,每个模型代表珍珠表面的6.3%。此外,我们还生成了一个代表珍珠表面10.3%的组合模型。这展示了使用适当仪器重建整个珍珠的潜力。我们强调,我们的方法不仅适用于珍珠和球形物体,还可以通过使用适当的采集设备适用于各种物体类型。我们提供了一个概念验证,证明了使用光学显微镜进行三维摄影测量的可行性。因此,我们的方法为在微观尺度上生成三维模型提供了一种实用且经济高效的替代方案,特别是在不需要详细内部结构信息的情况下。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/4940b9c3747e/41598_2024_64680_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/8ceaa1793914/41598_2024_64680_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/b700e74fb46e/41598_2024_64680_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/a72b4abc8bbd/41598_2024_64680_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/733ff03bcf4c/41598_2024_64680_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/febfa86ef8bd/41598_2024_64680_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/e00c85edfe37/41598_2024_64680_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/4940b9c3747e/41598_2024_64680_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/8ceaa1793914/41598_2024_64680_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/b700e74fb46e/41598_2024_64680_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/a72b4abc8bbd/41598_2024_64680_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/733ff03bcf4c/41598_2024_64680_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/febfa86ef8bd/41598_2024_64680_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/e00c85edfe37/41598_2024_64680_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2a/11186833/4940b9c3747e/41598_2024_64680_Fig7_HTML.jpg

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Assessment of statistical agreement of three techniques for the study of cut marks: 3D digital microscope, laser scanning confocal microscopy and micro-photogrammetry.三种用于切割痕迹研究的技术的统计一致性评估:三维数字显微镜、激光扫描共聚焦显微镜和显微摄影测量法。
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