IGEPP, INRAE, Institut Agro, Université de Rennes, 35650, Le Rheu, France.
Syngenta SA France, 1228 Chemin de L'Hobit, 31790, Saint-Sauveur, France.
Theor Appl Genet. 2024 Jun 19;137(7):164. doi: 10.1007/s00122-024-04664-3.
A comprehensive environmental characterization allowed identifying stable and interactive QTL for seed yield: QA09 and QC09a were detected across environments; whereas QA07a was specifically detected on the most stressed environments. A main challenge for rapeseed consists in maintaining seed yield while adapting to climate changes and contributing to environmental-friendly cropping systems. Breeding for cultivar adaptation is one of the keys to meet this challenge. Therefore, we propose to identify the genetic determinant of seed yield stability for winter oilseed rape using GWAS coupled with a multi-environmental trial and to interpret them in the light of environmental characteristics. Due to a comprehensive characterization of a multi-environmental trial using 79 indicators, four contrasting envirotypes were defined and used to identify interactive and stable seed yield QTL. A total of four QTLs were detected, among which, QA09 and QC09a, were stable (detected at the multi-environmental trial scale or for different envirotypes and environments); and one, QA07a, was specifically detected into the most stressed envirotype. The analysis of the molecular diversity at QA07a showed a lack of genetic diversity within modern lines compared to older cultivars bred before the selection for low glucosinolate content. The results were discussed in comparison with other studies and methods as well as in the context of breeding programs.
全面的环境特征分析可鉴定出稳定和互作的产量 QTL：QA09 和 QC09a 在多个环境中均被检测到；而 QA07a 仅在压力最大的环境中特异性检测到。油菜籽面临的主要挑战是在适应气候变化和促进环境友好型种植系统的同时保持产量。培育适应品种是应对这一挑战的关键之一。因此，我们建议使用 GWAS 结合多环境试验来鉴定冬油菜籽产量稳定性的遗传决定因素，并根据环境特征对其进行解释。由于使用 79 个指标对多环境试验进行了全面特征分析，因此定义了四个对比的生态型，并用于鉴定互作和稳定的产量 QTL。共检测到四个 QTL，其中 QA09 和 QC09a 是稳定的（在多环境试验规模或不同生态型和环境中检测到）；而 QA07a 则专门在压力最大的生态型中检测到。在 QA07a 处的分子多样性分析表明，与在低硫苷含量选择之前培育的较老品种相比，现代品种内的遗传多样性匮乏。研究结果与其他研究和方法进行了讨论，并结合了育种计划的背景进行了讨论。
