Department of Urology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, China.
Department of Gynaecological Oncology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, China.
J Cell Mol Med. 2024 Jun;28(12):e18475. doi: 10.1111/jcmm.18475.
Aurora kinase B (AURKB), an essential regulator in the process of mitosis, has been revealed through various studies to have a significant role in cancer development and progression. However, the specific mechanisms remain poorly understood. This study, therefore, seeks to elucidate the multifaceted role of AURKB in diverse cancer types. This study utilized bioinformatics techniques to examine the transcript, protein, promoter methylation and mutation levels of AURKB. The study further analysed associations between AURKB and factors such as prognosis, pathological stage, biological function, immune infiltration, tumour mutational burden (TMB) and microsatellite instability (MSI). In addition, immunohistochemical staining data of 50 cases of renal clear cell carcinoma and its adjacent normal tissues were collected to verify the difference in protein expression of AURKB in the two tissues. The results show that AURKB is highly expressed in most cancers, and the protein level of AURKB and the methylation level of its promoter vary among cancer types. Survival analysis showed that AURKB was associated with overall survival in 12 cancer types and progression-free survival in 11 cancer types. Elevated levels of AURKB were detected in the advanced stages of 10 different cancers. AURKB has a potential impact on cancer progression through its effects on cell cycle regulation as well as inflammatory and immune-related pathways. We observed a strong association between AURKB and immune cell infiltration, immunomodulatory factors, TMB and MSI. Importantly, we confirmed that the AURKB protein is highly expressed in kidney renal clear cell carcinoma (KIRC). Our study reveals that AURKB may be a potential biomarker for pan-cancer and KIRC.
极光激酶 B（AURKB）作为有丝分裂过程中的重要调节因子，已被多项研究揭示在癌症的发生和发展中具有重要作用。然而，其具体机制仍知之甚少。因此，本研究旨在阐明 AURKB 在多种癌症类型中的多方面作用。本研究利用生物信息学技术检测了 AURKB 的转录本、蛋白、启动子甲基化和突变水平。该研究还分析了 AURKB 与预后、病理分期、生物学功能、免疫浸润、肿瘤突变负荷（TMB）和微卫星不稳定性（MSI）等因素之间的关联。此外，收集了 50 例肾透明细胞癌及其相邻正常组织的免疫组织化学染色数据，以验证两种组织中 AURKB 蛋白表达的差异。结果表明，AURKB 在大多数癌症中呈高表达，且 AURKB 的蛋白水平及其启动子的甲基化水平在不同癌症类型中存在差异。生存分析表明，AURKB 与 12 种癌症的总生存以及 11 种癌症的无进展生存相关。在 10 种不同癌症的晚期阶段，检测到 AURKB 水平升高。AURKB 通过对细胞周期调控以及炎症和免疫相关途径的影响，对癌症进展具有潜在影响。我们观察到 AURKB 与免疫细胞浸润、免疫调节因子、TMB 和 MSI 之间存在强烈关联。重要的是，我们证实 AURKB 蛋白在肾透明细胞癌（KIRC）中高度表达。我们的研究表明，AURKB 可能是泛癌和 KIRC 的潜在生物标志物。
