Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
Physiol Plant. 2024 May-Jun;176(3):e14390. doi: 10.1111/ppl.14390.
A previously identified wheat drought stress responsive Universal stress protein, TaUSP_3B-1 has been found to work in an auxin dependent manner in the plant root tissues in the differentiation zone. We also found a novel interacting partner, TaGolS, which physically interacts with TaUSP_3B-1 and colocalizes in the endoplasmic reticulum. TaGolS is a key enzyme in the RFO (Raffinose oligosaccharides) biosynthesis which is well reported to provide tolerance under water deficit conditions. TaUSP_3B-1 overexpression lines showed an early flowering phenotype under drought stress which might be attributed to the increased levels of AtTPPB and AtTPS transcripts under drought stress. Moreover, at the cellular levels ER stress induced TaUSP_3B-1 transcription and provides tolerance in both adaptive and acute ER stress via less ROS accumulation in the overexpression lines. TaUSP_3B-1 overexpression plants had increased silique numbers and a denser root architecture as compared to the WT plants under drought stress.
先前鉴定的小麦干旱胁迫响应的泛素结合酶 TaUSP_3B-1 被发现以依赖生长素的方式在植物根组织的分化区起作用。我们还发现了一个新的互作伙伴 TaGolS，它与 TaUSP_3B-1 发生物理互作，并在内质网中共定位。TaGolS 是 RFO（棉子糖寡糖）生物合成中的关键酶，已有充分报道表明它在水分亏缺条件下提供耐受性。TaUSP_3B-1 过表达系在干旱胁迫下表现出早花表型，这可能归因于干旱胁迫下 AtTPPB 和 AtTPS 转录本水平的增加。此外，在细胞水平上，ER 应激诱导 TaUSP_3B-1 转录，并通过在过表达系中减少 ROS 积累，在适应性和急性 ER 应激中提供耐受性。与野生型植物相比，干旱胁迫下 TaUSP_3B-1 过表达植株的角果数量增加，根系结构更加密集。
