Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
Plant Cell Rep. 2023 Sep;42(9):1487-1501. doi: 10.1007/s00299-023-03043-4. Epub 2023 Jun 21.
TaUSPs are localized in Endoplasmic reticulum and form homo and hetero dimers within themselves. They play significant role in multiple abiotic stress responses in yeast heterologous system and in plants. Universal Stress Proteins are stress responsive proteins present in a variety of life forms ranging from bacteria to multicellular plants and animals. In this study we have identified 85 TaUSP genes in the wheat genome and have characterised their abiotic stress responsive members in yeast under different stress conditions. Localization and Y2H studies suggest that wheat, USP proteins are localized in the ER complex, and extensively crosstalk amongst themselves through forming hetero and homodimers. Expression analysis of these TaUSP genes suggests their role in adaptation to multiple abiotic stresses. TaUSP_5D-1 was found to have some DNA binding activity in yeast. Certain abiotic stress responsive TaUSP genes are found to impart tolerance to temperature stress, oxidative stress, ER stress (DTT treatment) and LiCl stress in the yeast heterologous system. TaUSP_5D-1 overexpression in A. thaliana imparts drought tolerance via better lateral root network in transgenic lines. The TaUSP represents an important repertoire of genes for engineering abiotic stress responsiveness in crop plants.
TaUSPs 定位于内质网，在自身内形成同型和异型二聚体。它们在酵母异源系统和植物中的多种非生物胁迫反应中发挥重要作用。普遍应激蛋白是存在于各种生命形式中的应激响应蛋白，从细菌到多细胞植物和动物。在这项研究中，我们在小麦基因组中鉴定了 85 个 TaUSP 基因，并在不同胁迫条件下在酵母中表征了它们的非生物胁迫响应成员。定位和 Y2H 研究表明，小麦 USP 蛋白定位于内质网复合物中，并通过形成异型和同型二聚体在彼此之间广泛交流。这些 TaUSP 基因的表达分析表明它们在适应多种非生物胁迫中的作用。在酵母中发现 TaUSP_5D-1 具有一定的 DNA 结合活性。某些非生物胁迫响应的 TaUSP 基因被发现能够在酵母异源系统中赋予对温度胁迫、氧化应激、内质网应激（DTT 处理）和 LiCl 应激的耐受性。在拟南芥中过表达 TaUSP_5D-1 通过转基因系中更好的侧根网络赋予耐旱性。TaUSP 代表了一个重要的基因库，用于工程作物植物的非生物胁迫响应。
