School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1, Payupnai, Wangchan, Rayong 21210, Thailand.
Department of Materials Science and Engineering, School of Molecular Science and Engineering (MSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1, Payupnai, Wangchan, Rayong 21210, Thailand.
Bioresour Technol. 2024 Aug;406:130945. doi: 10.1016/j.biortech.2024.130945. Epub 2024 Jun 18.
Described is chitinase immobilization on magnetic nanoparticles (MNPs) as biocompatible support for enzymatic production of di-N-acetyl chitobiose from chitin waste. Chitinase immobilization was feasible with an immobilization yield of 88.9 ± 1.6 % with 97.8 ± 1.0 % retention of activity and compared to free enzyme work, immobilization conferred better thermal and storage stability. As practical benefit the attachment to magnetic nanocarriers enabled easy enzyme recovery after repeated application runs and thus sustainable reuse. In fixed state chitinase retained a remarkable 39.7 ± 2.6 % of the starting activity after 16 reaction cycles. Furthermore, immobilized chitinase showed higher catalytic activity than free chitinase in converting shrimp shells and squid-pens chitins into di-N-acetyl chitobiose in a single-step reaction. The final yield of purified compound was 37.0 ± 1.2 % from shrimp shells and 61.1 ± 0.5 % from squid-pens chitin. In conclusion, an efficient MNP-based chitinase immobilization system with the potential for large-scale production was developed.
本文描述了壳聚糖酶在磁性纳米粒子（MNPs）上的固定化，将其作为生物相容性载体用于从壳聚糖废物中酶法生产二乙酰壳二糖。壳聚糖酶的固定化产率为 88.9±1.6%，活性保留率为 97.8±1.0%，与游离酶相比，固定化赋予了更好的热稳定性和储存稳定性。作为实际效益，将酶附着到磁性纳米载体上后，可在重复应用运行后轻松回收酶，从而实现可持续的重复使用。在固定状态下，壳聚糖酶在 16 次反应循环后仍保留了起始活性的 39.7±2.6%。此外，固定化壳聚糖酶在将虾壳和鱿鱼笔壳聚糖转化为二乙酰壳二糖的单步反应中显示出比游离壳聚糖酶更高的催化活性。从虾壳中得到的纯化产物的最终收率为 37.0±1.2%，从鱿鱼笔壳聚糖中得到的最终收率为 61.1±0.5%。总之，开发了一种基于磁性纳米粒子的高效壳聚糖酶固定化系统，具有大规模生产的潜力。
