Division of Chemistry I, Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.
Drug Research Program and Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Methods Mol Biol. 2024;2817:133-143. doi: 10.1007/978-1-0716-3934-4_11.
Nontargeted single-cell proteomics analysis by mass spectrometry with sample multiplexing utilizing isobaric labeling is often performed using a carrier proteome. The presented protocol describes a targeted approach that replaces the carrier proteome with a set of synthetic peptides from selected proteins, which improves the identification and quantification of these proteins in single human cells.
非靶向单细胞蛋白质组学分析通过质谱利用等压标记进行样品多重化分析通常使用载体蛋白质组。本方案描述了一种靶向方法，即用来自选定蛋白质的一组合成肽代替载体蛋白质组，从而提高了单个人类细胞中这些蛋白质的鉴定和定量。
