Department of Ophthalmology, Zunyi First People's Hospital, The Third Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Zunyi Medical University, Zunyi, China.
BMC Ophthalmol. 2024 Jun 21;24(1):267. doi: 10.1186/s12886-024-03523-5.
Diabetic retinopathy (DR) is a common complication of diabetes, and recent findings have shown that long noncoding RNAs (lncRNAs) may be involved in its pathogenesis. Through bioinformatics analysis, we found that lncRNA ATP2B2-IT2 may be involved in this process. This study primarily investigated the expression of the lncRNA ATP2B2-IT2 in human retinal microvascular endothelial cells (HRMECs) under high-glucose conditions and its effects on HRMEC proliferation, migration, and neovascularization.
We used RT‒PCR to assess the expression levels of lncRNA ATP2B2-IT2 and vascular endothelial growth factor (VEGF) in HRMECs under normal glucose (5.5 mmol/L) and high glucose (30 mmol/L) conditions. HRMECs were subsequently divided into four groups: the normal glucose (NG), high glucose (HG), high glucose with lncRNA ATP2B2-IT2 silencing (HG + si-lncRNA ATP2B2-IT2), and high glucose with silencing control (HG + si-NC) groups. The expression levels of the lncRNA ATP2B2-IT2 and VEGF in each group were determined using RT‒PCR. Thereafter, cell proliferation, migration, and neovascularization were assessed using CCK-8, Transwell, and tube formation assays, respectively.
RT‒PCR revealed that the expression levels of the lncRNA ATP2B2-IT2 and VEGF were greater in the HG group than in the NG group (P < 0.05). After silencing of the lncRNA ATP2B2-IT2, the expression of VEGF decreased significantly (P < 0.05). Subsequent CCK-8, Transwell, and tube formation assays demonstrated that compared to those in the NG group, the HRMECs in the HG group exhibited significantly increased proliferation, migration, and neovascularization (P < 0.05). However, after silencing of the lncRNA ATP2B2-IT2, the proliferation, migration, and neovascularization of HRMECs were significantly decreased in the HG + si-lncRNA ATP2B2-IT2 group compared to those in the HG group (P < 0.05).
LncRNA ATP2B2-IT2 may promote the proliferation, migration and neovascularization of HRMECs under high-glucose conditions.
糖尿病视网膜病变（DR）是糖尿病的一种常见并发症，最近的研究结果表明，长链非编码 RNA（lncRNA）可能参与其发病机制。通过生物信息学分析，我们发现 lncRNA ATP2B2-IT2 可能参与了这一过程。本研究主要探讨了 lncRNA ATP2B2-IT2 在高糖环境下人视网膜微血管内皮细胞（HRMEC）中的表达及其对 HRMEC 增殖、迁移和新生血管形成的影响。
采用 RT-PCR 检测正常葡萄糖（5.5mmol/L）和高葡萄糖（30mmol/L）条件下 HRMEC 中 lncRNA ATP2B2-IT2 和血管内皮生长因子（VEGF）的表达水平。随后将 HRMEC 分为正常葡萄糖组（NG）、高葡萄糖组（HG）、高葡萄糖加 lncRNA ATP2B2-IT2 沉默组（HG+si-lncRNA ATP2B2-IT2）和高葡萄糖加沉默对照组（HG+si-NC）。采用 RT-PCR 检测各组 lncRNA ATP2B2-IT2 和 VEGF 的表达水平。然后，采用 CCK-8 法、Transwell 法和管形成实验分别检测细胞增殖、迁移和新生血管形成。
RT-PCR 结果显示，HG 组 lncRNA ATP2B2-IT2 和 VEGF 的表达水平均高于 NG 组（P<0.05）。沉默 lncRNA ATP2B2-IT2 后，VEGF 的表达显著降低（P<0.05）。随后的 CCK-8 法、Transwell 法和管形成实验结果表明，与 NG 组相比，HG 组 HRMEC 的增殖、迁移和新生血管形成能力均显著增强（P<0.05）。然而，沉默 lncRNA ATP2B2-IT2 后，HG+si-lncRNA ATP2B2-IT2 组 HRMEC 的增殖、迁移和新生血管形成能力较 HG 组显著降低（P<0.05）。
在高糖条件下，lncRNA ATP2B2-IT2 可能促进 HRMEC 的增殖、迁移和新生血管形成。
