Zunyi Medical University, Zunyi, Guizhou, China.
Department of Ophthalmology, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, China.
BMC Ophthalmol. 2023 Mar 10;23(1):94. doi: 10.1186/s12886-023-02817-4.
Diabetic retinopathy (DR) is a leading cause of blindness. Vision threat is particularly severe in patients with retinal neovascularization. However, little is known about the role of long noncoding RNAs (lncRNAs) in proliferative diabetic retinopathy (PDR). The goal of this study was to identify lncRNAs involved in PDR.
We compared lncRNA expression profiles in the vitreous between patients with PDR and those with idiopathic macular hole (IMH) and between patients with PDR who had received anti-vascular endothelial growth factor (VEGF) therapy and those who had not. Vitreous samples from patients with PDR and IMH were screened for lncRNAs using microarray-based analysis, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to confirm the microarray results. Bioinformatic analysis was also performed. Moreover, the effect of anti-VEGF therapy was investigated in vitreous samples of patients with PDR treated with anti-VEGF therapy and those who were not.
A total of 1067 differentially expressed noncoding RNA transcripts were found during screening in the vitreous humor of patients with PDR than in those with IMH. Five lncRNAs were subjected to qRT-PCR. RP11-573 J24.1, RP11-787B4.2, RP11-654G14.1, RP11-2A4.3, and RP11-502I4.3 were significantly downregulated; this was validated by the comparison using the microarray data. In addition, 835 differentially expressed noncoding RNA transcripts were found during screening in the vitreous humor of patients with PDR treated with anti-VEGF therapy compared with untreated PDR patients. RP4-631H13.2 was significantly upregulated, which is consistent with the trend of the microarray analysis.
There were systemic expression differences in the vitreous at the microarray level between patients with PDR and those with IMH and between patients with PDR after anti-VEGF treatment and those that did not receive anti-VEGF treatment. LncRNAs identified in the vitreous humor may be a novel research field for PDR.
糖尿病视网膜病变（DR）是失明的主要原因。视网膜新生血管患者的视力威胁尤其严重。然而，关于长链非编码 RNA（lncRNA）在增生性糖尿病视网膜病变（PDR）中的作用知之甚少。本研究的目的是鉴定与 PDR 相关的 lncRNA。
我们比较了 PDR 患者和特发性黄斑裂孔（IMH）患者玻璃体中的 lncRNA 表达谱，以及接受抗血管内皮生长因子（VEGF）治疗和未接受抗 VEGF 治疗的 PDR 患者玻璃体中的 lncRNA 表达谱。使用基于微阵列的分析筛选 PDR 和 IMH 患者的玻璃体中的 lncRNA，并用实时定量聚合酶链反应（qRT-PCR）验证微阵列结果。还进行了生物信息学分析。此外，还研究了接受抗 VEGF 治疗的 PDR 患者玻璃体样本中抗 VEGF 治疗的效果。
在 PDR 患者玻璃体中与 IMH 患者相比，筛选出 1067 个差异表达的非编码 RNA 转录本。对 5 个 lncRNA 进行 qRT-PCR。RP11-573J24.1、RP11-787B4.2、RP11-654G14.1、RP11-2A4.3 和 RP11-502I4.3 明显下调，这与微阵列数据的比较结果一致。此外，与未经抗 VEGF 治疗的 PDR 患者相比，接受抗 VEGF 治疗的 PDR 患者玻璃体中筛选出 835 个差异表达的非编码 RNA 转录本。RP4-631H13.2 明显上调，与微阵列分析的趋势一致。
在 PDR 患者和 IMH 患者以及接受抗 VEGF 治疗和未接受抗 VEGF 治疗的 PDR 患者的玻璃体中，在微阵列水平上存在系统的表达差异。在玻璃体中鉴定的 lncRNA 可能是 PDR 的一个新的研究领域。
