Il Yelena, Il Dmitrii, Zabolotnykh Mikhail, Savenkova Inna, Nurzhanova Kulsara, Zhantleuov Daniyar, Kozhebayev Bolatpek, Akhmetova Balnur, Satiyeva Kaliya, Kurmangali Lailim
Department of Food Security, Agrotechnological Faculty, Manash Kozybaev North Kazakhstan University, Petropavlovsk, Kazakhstan.
Department of Veterinary and Sanitary Expertise of Livestock Product and Hygiene of Farm Animals, Faculty of Veterinary Medicine, P.A. Stolypin Omsk State Agrarian University, Omsk, Russian Federation.
Vet World. 2024 May;17(5):1130-1138. doi: 10.14202/vetworld.2024.1130-1138. Epub 2024 May 17.
Biochemical blood testing is the main diagnostic indicator of the clinical condition of highly productive animals and a method of determining changes in metabolic disorders. This study focuses on metabolic changes (ketosis), which are of the utmost importance in the assessment of the health status of animals, as well as differences in intergroup characteristics. The main focus of this study is to demonstrate the influence of subclinical ketosis in highly productive cows on changes in biochemical blood parameters during different physiological periods to further prevent this disease, adjust feeding rations, and prevent premature culling of animals. This study aimed to evaluate and establish changes in the biochemical status dynamics of highly productive cows with metabolic disorders in an industrial livestock complex.
Blood samples were systematically collected from highly productive cows of the Simmental breed (n = 60) and served as the primary material for subsequent analyses. Each methodological step was designed to ensure evaluation of the metabolic changes associated with post-calving adjustments in highly productive dairy cows. This study employed a comprehensive approach integrating clinical assessments, laboratory analyses, biochemical evaluations, instrumental measurements, and statistical analyses.
A biochemical blood test showed that the number of ketone bodies in the experimental group exceeded the norm, varied depending on the physiological state of the animals, and ranged from 0.89 to 1.45 mmol/L. At 10 days after calving, the highest indicator was 1.45 ± 0.05 mmol/L. This indicator was 1.05 mmol/L higher than that in the control group and exceeded the norm by 0.95.
Excess ketone bodies in the blood of animals led to accumulation in urine and milk, indicating a disturbance in metabolic processes in the body and a decrease in the quality of animal husbandry products. The sample size and the focus on a single breed from one geographical location may limit the generalizability of the findings. Further research should explore the mechanistic bases of ketosis development, potentially integrating genomic and proteomic approaches to understand the genetic predispositions and molecular pathways involved.
血液生化检测是高产动物临床状况的主要诊断指标，也是确定代谢紊乱变化的一种方法。本研究聚焦于代谢变化（酮病），其在评估动物健康状况以及组间特征差异方面至关重要。本研究的主要重点是证明高产奶牛亚临床酮病对不同生理时期血液生化参数变化的影响，以进一步预防该疾病、调整日粮，并防止动物过早淘汰。本研究旨在评估并确定工业畜牧场中患有代谢紊乱的高产奶牛生化状态动态变化。
从西门塔尔品种的高产奶牛（n = 60）中系统采集血液样本，作为后续分析的主要材料。每个方法步骤都旨在确保评估与高产奶牛产后调整相关的代谢变化。本研究采用了综合方法，包括临床评估、实验室分析、生化评估、仪器测量和统计分析。
血液生化检测显示，实验组酮体数量超过正常范围，随动物生理状态而异，范围为0.89至1.45 mmol/L。产犊后10天，最高指标为1.45 ± 0.05 mmol/L。该指标比对照组高1.05 mmol/L，超出正常范围0.95。
动物血液中酮体过多导致尿液和乳汁中积累，表明体内代谢过程紊乱，畜牧产品质量下降。样本量以及对来自一个地理位置的单一品种的关注可能会限制研究结果的普遍性。进一步的研究应探索酮病发生的机制基础，可能整合基因组学和蛋白质组学方法来了解其中涉及的遗传易感性和分子途径。
