Molecular Biology and Genetics Department, Faculty of Arts and Sciences, Uşak University, 64200, Uşak, Turkey.
Materials Science and Nanotechnology Engineering Department, Faculty of Engineering, Uşak University, 64200, Uşak, Turkey.
Bull Environ Contam Toxicol. 2020 Feb;104(2):215-221. doi: 10.1007/s00128-020-02783-3. Epub 2020 Jan 13.
Silicon nanoparticles gained a great interest due to its use in biomedical research. It is considered as safe and has been used in nanomedicine. But literature still states its toxicity depending upon the size and dose of silicon nanoparticles. So, current study was aimed to evaluate the cytotoxicity and genotoxicity of silicon dioxide nanoparticles (SiONPs) by Allium anaphase-telophase and Comet tests. Characterization of SiONPs showed the particle size as 16.12 ± 3.07 nm. The mean diameter of SiONPs was having range of 404.66 ± 93.39 nm in solution. Highest total anomalies (18.80 ± 0.45) were observed at 100 µg/mL, whereas least (11.2 ± 0.84) were observed by the 12.5 µg/mL concentration. There was concentration-response association in increased CAs and DNA damage. The highest concentration (100 µg/mL) of SiONPs induced the significant DNA damage (149.67 ± 1.15), whereas the least was observed by the negative control (2.67 ± 0.58). The current study revealed the cytotoxic and genotoxic effects of SiONPs on the root meristem cells of A. cepa.
由于其在生物医学研究中的应用，硅纳米颗粒引起了极大的关注。它被认为是安全的，并已被用于纳米医学。但是文献仍然根据硅纳米颗粒的大小和剂量来描述其毒性。因此，本研究旨在通过洋葱根尖细胞有丝分裂和彗星试验评估二氧化硅纳米颗粒（SiONPs）的细胞毒性和遗传毒性。SiONPs 的特性表明其粒径为 16.12 ± 3.07nm。SiONPs 的平均粒径在溶液中范围为 404.66 ± 93.39nm。在 100µg/mL 时观察到最高的总异常（18.80 ± 0.45），而在 12.5µg/mL 浓度时观察到最低的总异常（11.2 ± 0.84）。在增加的 CA 和 DNA 损伤中存在浓度-反应关联。SiONPs 的最高浓度（100µg/mL）诱导了显著的 DNA 损伤（149.67 ± 1.15），而阴性对照（2.67 ± 0.58）观察到的损伤最小。本研究揭示了 SiONPs 对洋葱根尖细胞有丝分裂细胞的细胞毒性和遗传毒性作用。
