Doctoral Program in Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
National Laboratory of Prof Sri Oemijati, Ministry of Health of the Republic of Indonesia, Jakarta, Indonesia.
BMC Res Notes. 2024 Jun 25;17(1):176. doi: 10.1186/s13104-024-06825-5.
Recent spoligotyping results in the island nation of Indonesia had revealed the existence of Mycobacterium tuberculosis complex lineage 3 (MTBC L3) or Central Asian (CAS) strains. In this work, whole-genome sequencing (WGS) - based methods were used to search for the presence of MTBC L3.
Two unrelated Indonesian L3 strains discovered by WGS-based SNP phylogenomics are presented here for the first time. Assemblies of their genomes yielded 96.95% (MTBC strain Mtb_S6970) and 98.35% (Mtb_S19106) of the known reference strain H37Rv. Their respective constructed genome coverages are 45.38 ± 12.95x and 63.13 ± 21.10x. The two L3 genomes have 4062 and 4121 genes, respectively, which are well within the number of genes predicted in MTBC strains. Instead of having three rRNA genes usually, Mtb_S6970 possesses four. These L3 isolates exhibit cross-class antibiotic susceptibility. FadD26, fadE24, fbpA, lprO, and panC, which are thought to be important in the pathophysiology of MTBC, were discovered to have 3-7 times more loci in L3 than L2 or L4. The penetration of L3 in the nation, despite its antibiotic sensitivity, is a concerning indicator of borderless global spread that may eventually be overcome by the phenotypes of acquired drug resistance.
印度尼西亚这个岛国最近的 spoligotyping 结果显示，存在结核分枝杆菌复合群 3 型（MTBC L3）或中亚（CAS）株。在这项工作中，使用全基因组测序（WGS）为基础的方法来寻找 MTBC L3 的存在。
本文首次介绍了两种通过 WGS 基于 SNP 系统发育基因组学发现的印度尼西亚 L3 无关株。它们的基因组组装分别获得了已知参考株 H37Rv 的 96.95%（MTBC 株 Mtb_S6970）和 98.35%（Mtb_S19106）。它们各自的基因组覆盖度分别为 45.38 ± 12.95x 和 63.13 ± 21.10x。这两个 L3 基因组分别有 4062 和 4121 个基因，分别在 MTBC 菌株中预测的基因数量范围内。Mtb_S6970 通常有三个 rRNA 基因，而有四个。这些 L3 分离株表现出交叉类抗生素敏感性。FadD26、fadE24、fbpA、lprO 和 panC 被认为在 MTBC 的病理生理学中很重要，它们在 L3 中的基因数量比 L2 或 L4 多 3-7 倍。尽管 L3 具有抗生素敏感性，但它在该国的渗透是一个令人担忧的指标，表明无国界的全球传播，最终可能会被获得性耐药性的表型所克服。
