School of Medical Engineering, Xinxiang Medical University, Xinxiang 453003, China.
Biosensors (Basel). 2024 May 21;14(6):261. doi: 10.3390/bios14060261.
Hydrogen peroxide (HO) is a signaling molecule that has the capacity to control a variety of biological processes in organisms. Cancer cells release more HO during abnormal tumor growth. There has been a considerable amount of interest in utilizing HO as a biomarker for the diagnosis of cancer tissue. In this study, an electrochemical sensor for HO was constructed based on 3D reduced graphene oxide (rGO), MXene (TiC), and multi-walled carbon nanotubes (MWCNTs) composite. Three-dimensional (3D) rGO-TiC-MWCNTs sensor showed good linearity for HO in the ranges of 1-60 μM and 60 μM-9.77 mM at a working potential of -0.25 V, with sensitivities of 235.2 µA mM cm and 103.8 µA mM cm, respectively, and a detection limit of 0.3 µM (S/N = 3). The sensor exhibited long-term stability, good repeatability, and outstanding immunity to interference. In addition, the modified electrode was employed to detect real-time HO release from cancer cells and cancer tissue ex vivo.
过氧化氢（HO）是一种信号分子，具有控制生物体各种生物过程的能力。癌细胞在异常肿瘤生长过程中会释放更多的 HO。人们对利用 HO 作为癌症组织诊断的生物标志物产生了浓厚的兴趣。在这项研究中，构建了一种基于 3D 还原氧化石墨烯（rGO）、MXene（TiC）和多壁碳纳米管（MWCNTs）复合材料的 HO 电化学传感器。在工作电位为-0.25 V 时，3D rGO-TiC-MWCNTs 传感器对 HO 的线性范围为 1-60 μM 和 60 μM-9.77 mM，灵敏度分别为 235.2 µA mM cm 和 103.8 µA mM cm，检测限为 0.3 µM（S/N = 3）。该传感器表现出长期稳定性、良好的可重复性和出色的抗干扰能力。此外，该修饰电极被用于检测癌细胞和离体癌症组织中实时的 HO 释放。
