Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China.
Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China.
Bioelectrochemistry. 2022 Dec;148:108225. doi: 10.1016/j.bioelechem.2022.108225. Epub 2022 Aug 6.
N-terminal pro-B-type natriuretic peptide is a major cardiac biomarker for early diagnosis and prognosis of heart failure. Herein, a novel label-free electrochemical immunosensor was developed based on home-made branched AuPd nanocrystals/N-doped porous carbon (AuPd NCS/NPC) for ultrasensitive and high-selective detection of N-terminal pro-B-type natriuretic peptide. Specifically, the AuPd NCS/NPC was prepared by a one-pot wet-chemical strategy by using thymine as a green structural directing agent, whose morphology, structures, and properties were strictly examined, showing high-efficiency catalysis towards electro-reduction of hydrogen peroxide. Under the optimized conditions, the fabricated sensor exhibited a dynamic linear range of 0.001 ∼ 10 ng mL and a low limit of detection (0.34 pg mL, S/N = 3) for immunoassay of N-terminal pro-B-type natriuretic peptide. Furthermore, this platform was explored for detection of the biomarker in human serum sample with satisfactory results. Thus, the built biosensor can render valuable guidance for prospective clinical diagnostic applications.
N-末端脑利钠肽前体是心力衰竭早期诊断和预后的主要心脏生物标志物。在此，我们基于自制的支化 AuPd 纳米晶/N-掺杂多孔碳 (AuPd NCS/NPC) 开发了一种新型无标记电化学免疫传感器，用于超灵敏和高选择性检测 N-末端脑利钠肽前体。具体而言，通过使用胸腺嘧啶作为绿色结构导向剂的一锅湿化学策略制备了 AuPd NCS/NPC，对其形态、结构和性能进行了严格的考察，显示出对过氧化氢电还原的高效催化作用。在优化条件下，制备的传感器对 N-末端脑利钠肽前体的免疫分析表现出 0.001~10ngmL 的动态线性范围和 0.34pgmL 的低检测限 (0.34pgmL，S/N=3)。此外，该平台还用于检测人血清样本中的生物标志物，结果令人满意。因此，所构建的生物传感器可为未来的临床诊断应用提供有价值的指导。
