Blood Purification Centre, Chun'an First People's Hospital, Zhejiang Provincial People's Hospital Chun'an Branch, Hangzhou Medical College Affiliated Chun'an Hospital, Hangzhou, Zhejiang, P.R. China.
Department of Emergency, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, P.R. China.
Biotechnol Appl Biochem. 2023 Jun;70(3):1024-1034. doi: 10.1002/bab.2417. Epub 2022 Dec 14.
A highly sensitive electrochemical biosensor was manufactured with triple synergistic catalysis to detect hydrogen peroxide (H O ). In this study, a highly sensitive biosensor based on Prussian blue-chitosan/graphene-hemin nanomaterial/platinum and palladium nanoparticles (PB-CS/HGNs/Pt&Pd biosensor) was fabricated for the detection of H O . The materials described above were modified on the electrode surface and applied to catalyze the breakdown of hydrogen peroxide. The current response of the biosensor presented a linear relationship with H O concentration from 6 × 10 to 20 μM (R = 0.9766) and with the logarithm of H O concentration from 20 to 9×10 μM (R = 0.9782), the low detection limit of 25 nM was obtained at the signal/noise (S/N) ratio of 3. Besides, the biosensor showed an outstanding anti-interference ability and acceptable reproducibility. PB-CS/HGNs/Pt&Pd electrodes are effective in measuring H O from living tumor cells, which implies that the biosensor has the potential to assess reactive oxygen species in various living tumor cells.
一种具有三重协同催化作用的高灵敏度电化学生物传感器被制造出来，用于检测过氧化氢（H₂O₂）。在这项研究中，基于普鲁士蓝-壳聚糖/石墨烯-血红素纳米材料/铂和钯纳米粒子（PB-CS/HGNs/Pt&Pd 生物传感器）制造了一种高灵敏度的生物传感器，用于检测 H₂O₂。上述材料被修饰在电极表面，并应用于催化过氧化氢的分解。生物传感器的电流响应与 H₂O₂浓度在 6×10 到 20 μM 之间呈现线性关系（R = 0.9766），与 H₂O₂浓度的对数在 20 到 9×10 μM 之间呈现线性关系（R = 0.9782），在信噪比为 3 时获得了 25 nM 的低检测限。此外，该生物传感器表现出出色的抗干扰能力和可接受的重现性。PB-CS/HGNs/Pt&Pd 电极在测量活肿瘤细胞中的 H₂O₂方面非常有效，这意味着该生物传感器有可能评估各种活肿瘤细胞中的活性氧物质。
