Wang Yucheng, Chang Yawen, Gong Weirong, Du Yuzhou
College of Plant Protection, Yangzhou University, Yangzhou 225000, China.
Plant Protection and Quarantine Station of Jiangsu Province, Nanjing 210036, China.
Insects. 2024 Jun 20;15(6):462. doi: 10.3390/insects15060462.
is a significant invasive pest that targets horticultural and vegetable crops, causing large-scale outbreaks characterized by pronounced thermotolerance and insecticide resistance. This study examined the impact of long-term selection for abamectin resistance during the larval stage of on its population dynamics and thermal tolerance. We conducted a comprehensive comparison between the abamectin-resistant strain (AB-R) and the susceptible strain (S), including age-stage, two-sex life table analysis, thermal preference (T), critical thermal maximum (CT), heat knockdown times (HKDTs), eclosion and survival rates, and expression under heat stress. Our results showed that while selection for abamectin resistance was detrimental to survival and reproduction, it activated self-defense mechanisms and rapid adaptive adjustments and conferred modest thermal tolerance, which suggests a dual nature of insecticide effects. The AB-R strain exhibited significantly higher thermal preference and CT values, along with a longer HKDT and improved survival. Additionally, there was a significant upregulation of expression in the AB-R strain compared to the S strain. These findings indicate that the evolution of thermal adaptation was accompanied by abamectin resistance development, emphasizing the necessity of considering temperature effects when applying chemical control. Our study provides valuable insights into how physiological acclimation may help mitigate the toxic effects of insecticides and illustrate how insects respond to multiple environmental pressures.
是一种重要的入侵害虫，以园艺作物和蔬菜作物为目标，引发大规模爆发，其特征为具有显著的耐热性和抗药性。本研究考察了在其幼虫阶段长期选择阿维菌素抗性对其种群动态和耐热性的影响。我们对阿维菌素抗性品系（AB-R）和敏感品系（S）进行了全面比较，包括年龄-阶段、两性生命表分析、热偏好（T）、临界热最大值（CT）、热击倒时间（HKDTs）、羽化率和存活率，以及热应激下的 表达。我们的结果表明，虽然选择阿维菌素抗性对生存和繁殖不利，但它激活了自我防御机制和快速适应性调整，并赋予了适度的耐热性，这表明杀虫剂效应具有双重性质。AB-R 品系表现出显著更高的热偏好和 CT 值，以及更长的 HKDT 和更高的存活率。此外，与 S 品系相比，AB-R 品系中的 表达显著上调。这些发现表明，热适应性的进化伴随着阿维菌素抗性的发展，强调了在应用化学防治时考虑温度效应的必要性。我们的研究为生理适应如何有助于减轻杀虫剂的毒性作用提供了有价值的见解，并说明了昆虫如何应对多种环境压力。
