College of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, 225000, China.
College of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, 225000, China.
J Therm Biol. 2024 Oct;125:103988. doi: 10.1016/j.jtherbio.2024.103988. Epub 2024 Sep 28.
The invasive pest, Liriomyza trifolii, poses a significant threat to ornamental and vegetable plants. It spreads rapidly and causes large-scale outbreaks with pronounced thermotolerance. In this study, we developed L. trifolii strains adapted to high temperatures (strains designated 35 and 40); these were generated from a susceptible strain (designated S) by long-term thermal acclimation to 35 °C and 40 °C, respectively. Age-stage, two-sex life tables, thermal preferences, critical thermal limits, knockdown behaviors, eclosion and survival rates as well as expression of genes encoding heat shock proteins (Hsps) were compared for the three strains. Our findings indicated that the thermotolerance of L. trifolii was enhanced after long-term thermal acclimation, which suggested an adaptive plastic response to thermal stress. A trade-off between reproduction and thermotolerance was observed under thermal stress, potentially improving survival of the population and fostering adaptionary changes. Acclimation at 35 °C improved reproductive performance and population density of L. trifolii, particularly by enhancing the fecundity of female adults and accelerating the speed of development. Although the 40 strain exhibited the highest developmental speed and greater thermotolerance, it incurred a larger reproductive cost. This study provides a theoretical framework for monitoring and controlling leafminers and understanding their evolutionary adaptation to environmental changes.
入侵害虫三叶斑潜蝇对观赏植物和蔬菜植物构成重大威胁。它传播迅速，具有明显的耐热性，会引发大规模爆发。在这项研究中，我们开发了适应高温的三叶斑潜蝇菌株（分别命名为 35 和 40 菌株）；这些菌株是通过长期在 35°C 和 40°C 下热驯化，从敏感菌株（命名为 S 菌株）中产生的。我们比较了这三个菌株的龄期-两性生命表、热偏好、临界热极限、击倒行为、羽化和存活率以及编码热休克蛋白（Hsps）的基因表达。研究结果表明，三叶斑潜蝇的耐热性在长期热驯化后得到增强，这表明对热应激具有适应性可塑性反应。在热应激下，繁殖和耐热性之间存在权衡，这可能提高了种群的生存能力，并促进了适应性变化。35°C 的驯化提高了三叶斑潜蝇的繁殖性能和种群密度，特别是通过提高雌成虫的生殖力和加速发育速度。尽管 40 菌株表现出最高的发育速度和更强的耐热性，但它的生殖成本更大。这项研究为监测和控制潜叶蝇以及理解它们对环境变化的进化适应提供了理论框架。
