Wang Yu-Cheng, Chang Ya-Wen, Du Yu-Zhou
Yangzhou University, College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou, China.
Yangzhou University, Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China.
PeerJ. 2021 Aug 31;9:e12064. doi: 10.7717/peerj.12064. eCollection 2021.
is an invasive pest of horticultural and vegetable crops that possesses robust competitive advantages that enable it to replace closely-related species. High temperatures often occur concomitantly with insecticide usage during outbreaks. In this study, we compared the transcriptomes of exposed to high temperature (40 °C T40), insecticide (LC of technical grade abamectin, I50) and combined high temperature and abamectin exposure (IT5040, I50 followed by T40; and TI4050, T40 followed by I50). RNA-seq generated and revealed 44,633 unigenes with annotation data; these were compared with COG and KEGG databases for functional classification and enrichment analysis. Compared with the I50 treatment, COG classification indicated that 'post-translational modification, protein turnover, chaperones' was enriched in the IT5040 treatment. In the TI4050 treatment, 'carbohydrate transport and metabolism' was the most abundant group. The most enriched KEGG pathways in the TI4050 and IT5040 treatments were 'longevity regulating pathway - multiple species' and 'protein processing in endoplasmic reticulum', respectively. Subsequent annotation and enrichment analyses indicated that stress-related genes such as and were differentially expressed in the I50 vs. TI4050 or I50 vs. IT5040 treatment groups. Three commercial insecticide formulations were also used to further verify the expression of selected differentially-expressed genes. This study will be conductive to consider the temperature effect on insecticide tolerance in , and provides a framework for improving the application efficiency of insecticides in hot weather, which will ultimately reduce the overuse of pesticides.
是园艺和蔬菜作物的一种入侵性害虫，具有强大的竞争优势，使其能够取代近缘物种。在害虫爆发期间，高温常常与杀虫剂的使用同时出现。在本研究中，我们比较了暴露于高温（40°C，T40）、杀虫剂（技术级阿维菌素的LC，I50）以及高温和阿维菌素联合暴露（IT5040，先I50后T40；和TI4050，先T40后I50）下的转录组。RNA测序产生并揭示了44,633个具有注释数据的单基因；将这些单基因与COG和KEGG数据库进行比较以进行功能分类和富集分析。与I50处理相比，COG分类表明“翻译后修饰、蛋白质周转、伴侣蛋白”在IT5040处理中富集。在TI4050处理中，“碳水化合物运输和代谢”是最丰富的类别。TI4050和IT5040处理中最富集的KEGG途径分别是“长寿调节途径 - 多种物种”和“内质网中的蛋白质加工”。随后的注释和富集分析表明，诸如[此处原文缺失相关基因名称]等与应激相关的基因在I50与TI4050或I50与IT5040处理组中差异表达。还使用了三种市售杀虫剂配方来进一步验证所选差异表达基因的表达。本研究将有助于考虑温度对[此处原文缺失害虫名称]杀虫剂耐受性的影响，并为提高炎热天气下杀虫剂的应用效率提供框架，最终减少农药的过度使用。
