Wang Yu-Cheng, Chang Ya-Wen, Bai Jing, Zhang Xiao-Xiang, Iqbal Junaid, Lu Ming-Xing, Gong Wei-Rong, Du Yu-Zhou
College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China.
Plant Protection and Quarantine Station of Jiangsu Province, Nanjing, China.
Ecotoxicol Environ Saf. 2021 Jul 15;218:112307. doi: 10.1016/j.ecoenv.2021.112307. Epub 2021 May 6.
The leafminer fly, Liriomyza trifolii, is an invasive pest of horticultural and vegetable crops that possesses a robust competitive ability when compared to congeneric species, especially with respect to temperature and insecticide tolerance. Abamectin, which is commonly used to control L. trifolii in the field, was selected as the target insecticide in this study. Our objective was to study the effect of abamectin and high temperature stress on L. trifolii mortality and the expression of genes encoding cytochrome P450 (CYP450s) and heat shock proteins (Hsps) by quantitative real-time reverse transcriptase PCR (qRT-PCR). When L. trifolii was exposed to abamectin followed by exposure to 40 °C (LC50 +HT40), mortality showed a significant increase, whereas exposure to 40 ℃ followed by abamectin (HT40+LC50) reduced mortality relative to abamectin or HT40 alone. Expression of three CYP450s in the CYP4 family was highest in the HT40+LC50 treatment, followed by the LC50+HT40 treatment. The expression levels of CYP18A1 (CYP18 family) were not significantly different among treatments, and CYP301A1 (CYP301 family) was only sensitive to temperature (HT40). The expression of five sHsps showed similar expression patterns and were highly responsive to the LC50+HT40 treatment, followed by the HT40 and HT40+LC50 treatments. Based on CYP450s and Hsps expression levels, our findings that suggest that L. trifolii exhibits adaptive cross-tolerance to high temperature and abamectin. This study provides a framework for selecting the most effective application time for abamectin with respect to controlling L. trifolii, which will ultimately reduce the overuse of pesticides.
斑潜蝇，即三叶草斑潜蝇（Liriomyza trifolii），是园艺作物和蔬菜作物的一种入侵性害虫，与同属物种相比具有很强的竞争能力，尤其是在温度和杀虫剂耐受性方面。阿维菌素是田间常用的防治三叶草斑潜蝇的药剂，本研究选择其作为目标杀虫剂。我们的目的是通过定量实时逆转录聚合酶链反应（qRT-PCR）研究阿维菌素和高温胁迫对三叶草斑潜蝇死亡率以及细胞色素P450（CYP450s）和热休克蛋白（Hsps）编码基因表达的影响。当三叶草斑潜蝇先接触阿维菌素后再暴露于40℃（LC50 + HT40）时，死亡率显著增加，而先暴露于40℃再接触阿维菌素（HT40 + LC50），相对于单独使用阿维菌素或HT40，死亡率降低。CYP4家族中三个CYP450s的表达在HT40 + LC50处理中最高，其次是LC50 + HT40处理。CYP18A1（CYP18家族）的表达水平在各处理间无显著差异，CYP301A1（CYP301家族）仅对温度（HT40）敏感。五个小分子热休克蛋白（sHsps）的表达呈现相似模式，对LC50 + HT40处理反应强烈，其次是HT40和HT40 + LC50处理。基于CYP450s和Hsps的表达水平，我们的研究结果表明三叶草斑潜蝇对高温和阿维菌素表现出适应性交叉耐受性。本研究为选择防治三叶草斑潜蝇的阿维菌素最有效施用时间提供了框架，这最终将减少农药的过度使用。
