大肠杆菌ML308 - 225中外膜蛋白的生物合成与周转
Biosynthesis and turnover of outer-membrane proteins in Escherichia coli ML308-225.
作者信息
Allen R J, Scott G K
出版信息
Biochem J. 1979 Aug 15;182(2):407-12. doi: 10.1042/bj1820407.
Abstract
Isolated outer membranes and outer-membrane extracts from Escherichia coli ML308-225 in the early-exponential growth phase contain more protein than do corresponding preparations from late-exponential- or stationary-phase bacteria. Isotope-dilution experiments show that this is due to a loss of protein from the membrane during the exponential growth phase. Inhibition of bacterial growth and protein synthesis stabilizes the outer-membrane-protein concentration. Protein synthesis in the absence of bacterial growth results in higher concentrations of protein in the outer membrane.
摘要
处于指数生长早期的大肠杆菌 ML308 - 225 的分离外膜和外膜提取物比处于指数生长后期或稳定期细菌的相应制剂含有更多蛋白质。同位素稀释实验表明，这是由于在指数生长阶段膜中的蛋白质流失所致。抑制细菌生长和蛋白质合成可稳定外膜蛋白浓度。在没有细菌生长的情况下进行蛋白质合成会导致外膜中蛋白质浓度升高。
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