大肠杆菌ML308 225及血清抗性突变体的外膜成分分析。
Analysis of outer membrane components of Escherichia coli ML308 225 and of a serum-resistant mutant.
作者信息
Tee G L, Scott G K
出版信息
Infect Immun. 1980 May;28(2):387-92. doi: 10.1128/iai.28.2.387-392.1980.
Abstract
Escherichia coli ML308 225 in the early exponential phase of batch cultures was killed by human serum in a complement-dependent reaction. Under the same conditions, a mutant derived from this strain by multiple subcultures in human serum was resistant to killing by serum. The components of the outer membrane of both the parent and mutant strains were analyzed. The protein and phospholipid analyses were identical, but the mutant strain produced twice as much lipopolysaccharide as the parent strain. These results suggest that the extent of bacterial surface coverage by lipopolysaccharide determines the degree of sensitivity to serum.
摘要
在分批培养的指数生长期早期的大肠杆菌ML308 225，在补体依赖反应中被人血清杀死。在相同条件下，通过在人血清中多次传代从该菌株衍生的突变体对血清杀伤具有抗性。对亲本菌株和突变体菌株的外膜成分进行了分析。蛋白质和磷脂分析结果相同，但突变体菌株产生的脂多糖是亲本菌株的两倍。这些结果表明，脂多糖对细菌表面的覆盖程度决定了对血清的敏感程度。
相似文献
1
Analysis of outer membrane components of Escherichia coli ML308 225 and of a serum-resistant mutant.大肠杆菌ML308 225及血清抗性突变体的外膜成分分析。
Infect Immun. 1980 May;28(2):387-92. doi: 10.1128/iai.28.2.387-392.1980.
2
Serum-resistant mutants of Escherichia coli O111 contain increased lipopolysaccharide, lack an O antigen-containing capsule, and cover more of their lipid A core with O antigen.大肠杆菌O111的血清抗性突变体含有增加的脂多糖，缺乏含O抗原的荚膜，并且其脂质A核心更多地被O抗原覆盖。
J Bacteriol. 1984 Sep;159(3):877-82. doi: 10.1128/jb.159.3.877-882.1984.
3
The effect of purified lipopolysaccharide on the bactericidal reaction of human serum complement.纯化脂多糖对人血清补体杀菌反应的影响。
J Gen Microbiol. 1980 Mar;117(1):65-72. doi: 10.1099/00221287-117-1-65.
4
Mechanism of bacterial resistance to complement-mediated killing: inserted C5b-9 correlates with killing for Escherichia coli O111B4 varying in O-antigen capsule and O-polysaccharide coverage of lipid A core oligosaccharide.细菌对补体介导杀伤的抗性机制：插入的C5b-9与不同O抗原荚膜、脂质A核心寡糖O-多糖覆盖情况的大肠杆菌O111B4的杀伤作用相关。
Infect Immun. 1984 Jul;45(1):113-7. doi: 10.1128/iai.45.1.113-117.1984.
5
Stable insertion of C5b-9 complement complexes into the outer membrane of serum treated, susceptible Escherichia coli cells as a prerequisite for killing.C5b-9补体复合物稳定插入经血清处理的易感大肠杆菌细胞外膜是杀菌的前提条件。
Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Dec;258(2-3):316-26. doi: 10.1016/s0176-6724(84)80050-4.
6
Human serum complement requirements for bacterial killing and protoplast lysis of Escherichia coli ML308 225.人血清补体对大肠杆菌ML308 225的细菌杀伤和原生质体裂解的需求
J Gen Microbiol. 1981 Mar;123(1):179-81. doi: 10.1099/00221287-123-1-179.
7
Superoxide dismutase protects Escherichia coli against killing by human serum.
Arch Biochem Biophys. 1995 Feb 20;317(1):57-61. doi: 10.1006/abbi.1995.1135.
8
Membrane changes induced by exposure of Escherichia coli to human serum.大肠杆菌暴露于人体血清所诱导的膜变化。
Infect Immun. 1983 Dec;42(3):1055-66. doi: 10.1128/iai.42.3.1055-1066.1983.
9
Interaction of human complement proteins with serum-sensitive and serum-resistant strains of Escherichia coli.人补体蛋白与大肠杆菌血清敏感株和血清耐受株的相互作用。
Mol Immunol. 1984 Jul;21(7):609-20. doi: 10.1016/0161-5890(84)90046-4.
10
Genetical studies of serum resistance in Escherichia coli.大肠杆菌血清抗性的遗传学研究。
J Gen Microbiol. 1975 Jul;89(1):57-66. doi: 10.1099/00221287-89-1-57.
引用本文的文献
1
Phenotypic heterogeneity enables uropathogenic Escherichia coli to evade killing by antibiotics and serum complement.表型异质性使尿路致病性大肠杆菌能够逃避抗生素和血清补体的杀伤作用。
Infect Immun. 2015 Mar;83(3):1056-67. doi: 10.1128/IAI.02725-14. Epub 2015 Jan 5.
2
Further characterization of complement resistance conferred on Escherichia coli by the plasmid genes traT of R100 and iss of ColV,I-K94.对R100的质粒基因traT和ColV、I-K94的iss赋予大肠杆菌的补体抗性进行进一步表征。
Infect Immun. 1982 Feb;35(2):654-9. doi: 10.1128/iai.35.2.654-659.1982.
3
Comparison of the effects of different lipopolysaccharides on the serum bactericidal reactions of two strains of Escherichia coli.不同脂多糖对两株大肠杆菌血清杀菌反应影响的比较
Infect Immun. 1981 Feb;31(2):831-2. doi: 10.1128/iai.31.2.831-832.1981.
4
Vwa+ phenotype of Yersinia enterocolitica.小肠结肠炎耶尔森菌的Vwa+表型
Infect Immun. 1983 Apr;40(1):166-71. doi: 10.1128/iai.40.1.166-171.1983.
5
Importance of complement source in bactericidal activity of human antibody and murine monoclonal antibody to meningococcal group B polysaccharide.补体来源在人源抗体及抗B群脑膜炎球菌多糖鼠单克隆抗体杀菌活性中的重要性
Infect Immun. 1983 Apr;40(1):257-64. doi: 10.1128/iai.40.1.257-264.1983.
6
Serum-resistant mutants of Escherichia coli O111 contain increased lipopolysaccharide, lack an O antigen-containing capsule, and cover more of their lipid A core with O antigen.大肠杆菌O111的血清抗性突变体含有增加的脂多糖，缺乏含O抗原的荚膜，并且其脂质A核心更多地被O抗原覆盖。
J Bacteriol. 1984 Sep;159(3):877-82. doi: 10.1128/jb.159.3.877-882.1984.
7
Inhibition of serum bactericidal reaction by lipopolysaccharide.脂多糖对血清杀菌反应的抑制作用。
Infect Immun. 1985 Jun;48(3):759-62. doi: 10.1128/iai.48.3.759-762.1985.
8
Plasmid-associated resistance of Salmonella typhimurium to complement activated by the classical pathway.鼠伤寒沙门氏菌的质粒相关抗性对经典途径激活的补体的抗性。
Infect Immun. 1987 Nov;55(11):2645-52. doi: 10.1128/iai.55.11.2645-2652.1987.
本文引用的文献
1
EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI.温度对大肠杆菌脂肪酸组成的影响
J Bacteriol. 1962 Dec;84(6):1260-7. doi: 10.1128/jb.84.6.1260-1267.1962.
2
3
KINETICS OF THE BACTERICIDAL ACTION OF NORMAL SERUM ON GRAM-NEGATIVE BACTERIA.正常血清对革兰氏阴性菌杀菌作用的动力学
J Immunol. 1965 Jul;95:75-9.
4
Lysis of gram-negative bacteria by serum.血清对革兰氏阴性菌的溶解作用。
J Gen Microbiol. 1958 Apr;18(2):529-33. doi: 10.1099/00221287-18-2-529.
5
The effect of purified lipopolysaccharide on the bactericidal reaction of human serum complement.纯化脂多糖对人血清补体杀菌反应的影响。
J Gen Microbiol. 1980 Mar;117(1):65-72. doi: 10.1099/00221287-117-1-65.
6
7
Synthesis and assembly of bacterial membrane components. A lipopolysaccharide-phospholipid-protein complex excreted by living bacteria.细菌膜成分的合成与组装。一种由活细菌分泌的脂多糖 - 磷脂 - 蛋白质复合物。
J Mol Biol. 1969 Sep 28;44(3):477-92. doi: 10.1016/0022-2836(69)90374-x.
8
Outer membrane proteins of Escherichia coli. IV. Differences in outer membrane proteins due to strain and cultural differences.大肠杆菌的外膜蛋白。IV. 因菌株和培养差异导致的外膜蛋白差异。
J Bacteriol. 1974 May;118(2):454-64. doi: 10.1128/jb.118.2.454-464.1974.
9
Alterations in the outer membrane of the cell envelope of heptose-deficient mutants of Escherichia coli.大肠杆菌庚糖缺陷型突变体细胞包膜外膜的改变。
J Bacteriol. 1974 Feb;117(2):527-43. doi: 10.1128/jb.117.2.527-543.1974.
10
Metabolically controlled killing of Pasteurella septica by antibody and complement.抗体和补体对败血巴斯德氏菌的代谢控制杀伤作用
Biochim Biophys Acta. 1974 Oct 8;362(3):598-601. doi: 10.1016/0304-4165(74)90157-3.
Zotero 插件