College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.
School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
PLoS One. 2024 Jun 26;19(6):e0304453. doi: 10.1371/journal.pone.0304453. eCollection 2024.
Hirudo nipponia is an important medicinal animal in China. Its salivary gland secretions contain a variety of protein bioactive substances. Investigations of its salivary glands are of great significance in the study of the medicinal value and mechanism of leech secretions. Illumina RNA-Seq technology was used to perform transcriptome sequencing of salivary gland tissue of H. nipponia under starvation (D30) and fed (D0) states. A total of 2,650 differentially expressed genes (DEGs) were screened. Using the label-free protein quantification technique and bioinformatics analysis, the expression of differentially expressed proteins (DEPs) in the salivary gland tissue of H. nipponia was compared. A total of 2,021 proteins were identified, among which 181 proteins were differentially expressed between the starvation and fed states, with 72 significantly upregulated and 109 significantly downregulated. The salivary glands of H. nipponia synthesized protein-based active substances after 30 days of starvation and adapted to the starvation environment by weakening respiratory activity and reducing metabolic activity to reduce energy expenditure. Energy was produced by glycolysis and the tricarboxylic acid cycle for the synthesis of substances such as antibiotics. This study combined transcriptome and proteome sequencing data to provide a data reference for an in-depth study of the regulatory mechanism of salivary gland secretions of H. nipponia under starvation stress by analyzing DEGs and DEPs.
日本医蛭是中国重要的药用动物，其唾液腺分泌多种具有生物活性的蛋白物质。研究其唾液腺对于研究医蛭分泌液的药用价值和作用机制具有重要意义。本研究采用 Illumina RNA-Seq 技术对饥饿（D30）和投喂（D0）状态下日本医蛭唾液腺组织进行转录组测序，共筛选到 2650 个差异表达基因（DEGs）。采用无标记蛋白定量技术和生物信息学分析方法，比较了日本医蛭唾液腺组织中差异表达蛋白（DEPs）的表达情况，共鉴定到 2021 个蛋白，其中饥饿和投喂状态下有 181 个蛋白差异表达，有 72 个蛋白表达上调，109 个蛋白表达下调。日本医蛭在饥饿 30 天后通过合成蛋白类活性物质来适应饥饿环境，减弱呼吸活性，降低代谢活性，减少能量消耗，同时通过糖酵解和三羧酸循环产生能量来合成抗生素等物质。本研究结合转录组和蛋白质组测序数据，通过分析 DEGs 和 DEPs，为深入研究日本医蛭在饥饿胁迫下唾液腺分泌液的调控机制提供了数据参考。
