State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Int J Mol Sci. 2024 Jun 8;25(12):6365. doi: 10.3390/ijms25126365.
In most cases, the number of honeybee stings received by the body is generally small, but honeybee stings can still cause serious allergic reactions. This study fully simulated bee stings under natural conditions and used H Nuclear Magnetic Resonance (H NMR) to analyze the changes in the serum metabolome of Sprague-Dawley (SD) rats stung once or twice by honeybees to verify the impact of this mild sting on the body and its underlying mechanism. The differentially abundant metabolites between the blank control rats and the rats stung by honeybees included four amino acids (aspartate, glutamate, glutamine, and valine) and four organic acids (ascorbic acid, lactate, malate, and pyruvate). There was no separation between the sting groups, indicating that the impact of stinging once or twice on the serum metabolome was similar. Using the Principal Component Discriminant Analysis ( PCA-DA) and Variable Importance in Projection (VIP) methods, glucose, lactate, and pyruvate were identified to help distinguish between sting groups and non-sting groups. Metabolic pathway analysis revealed that four metabolic pathways, namely, the tricarboxylic acid cycle, pyruvate metabolism, glutamate metabolism, and alanine, aspartate, and glutamate metabolism, were significantly affected by bee stings. The above results can provide a theoretical basis for future epidemiological studies of bee stings and medical treatment of patients stung by honeybees.
在大多数情况下，人体所受到的蜜蜂蜇刺数量通常较少，但蜜蜂蜇刺仍可能引起严重的过敏反应。本研究充分模拟了自然条件下的蜜蜂蜇刺，采用 1H 核磁共振（1H NMR）技术分析了蜜蜂蜇刺 1 次和 2 次的 Sprague-Dawley（SD）大鼠血清代谢组的变化，以验证这种轻度蜇刺对机体的影响及其潜在机制。与空白对照组大鼠相比，蜜蜂蜇刺组大鼠血清中差异丰度的代谢物包括 4 种氨基酸（天冬氨酸、谷氨酸、谷氨酰胺和缬氨酸）和 4 种有机酸（抗坏血酸、乳酸、苹果酸和丙酮酸）。蜇刺组之间没有分离，表明蜇刺 1 次和 2 次对血清代谢组的影响相似。利用主成分判别分析（PCA-DA）和变量重要性投影（VIP）方法，鉴定出葡萄糖、乳酸和丙酮酸有助于区分蜇刺组和非蜇刺组。代谢通路分析显示，有 4 条代谢通路，即三羧酸循环、丙酮酸代谢、谷氨酸代谢和丙氨酸、天冬氨酸和谷氨酸代谢，受到蜜蜂蜇刺的显著影响。上述结果可为未来的蜜蜂蜇刺流行病学研究和蜜蜂蜇伤患者的医疗提供理论依据。
