Granados-Amores Andrés, Campa-Córdova Ángel I, Acosta-Salmón Héctor, Angulo Carlos, Zenteno-Savín Tania, Rodríguez-Jaramillo Carmen, Saucedo Pedro E
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz 23096, Baja California Sur, Mexico.
Departamento Académico de Ingeniería en Pesquerías at Pichilingue, Universidad Autónoma de Baja California Sur at Pichilingue, La Paz 23000, Baja California Sur, Mexico.
Antioxidants (Basel). 2024 Jun 14;13(6):723. doi: 10.3390/antiox13060723.
To evaluate the antioxidant activity and oxidative damage by relaxing, wounding, and seeding of a saibo of different origin on hosts, five oyster treatments were included: (1) relaxed (REL) but neither wounded nor seeded; (2) relaxed and wounded (WOU) but not seeded; (3) relaxed, wounded, and seeded with an allograft (ALL); (4) relaxed, wounded, and seeded with an autograft (AUT); and (5) unrelaxed, unwounded, and unseeded as control (CTR). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and thiobarbituric acid (TBARS) activity were quantified between 3 and 24 h post-seeding. Compared to the CTR oysters, which did not suffer oxidative stress, SOD activity significantly decreased in the gonad and digestive gland in all treatments and decreased in mantle tissue in AUT oysters; this indicates that the entire process of preparing oysters for pearl culture (relaxing, wounding, and seeding) generates oxidative stress in the host. CAT was not a sensitive enzyme for measuring the short-term response of oysters to the wounding-seeding processes but rather a more prolonged or chronic stress. Similar to SOD, the lowest GPx and TBARS activity in seeded oysters evidenced their susceptibility to oxidative stress and damage, particularly in the WOU treatment. Evidence from this study indicates that SOD is a more sensitive enzyme for measuring the short-term response of the host oyster to the wounding and seeding of a saibo. It is also clear that the host undergoes stress at all stages of the pearl culture process, mostly during gonad wounding and regardless of the origin of saibo.
为了评估不同来源的赛博在宿主上进行松弛、创伤和接种后对其抗氧化活性和氧化损伤情况，设置了五种牡蛎处理方式：(1) 仅松弛（REL），未创伤也未接种；(2) 松弛且创伤（WOU），但未接种；(3) 松弛、创伤并接种同种异体移植物（ALL）；(4) 松弛、创伤并接种自体移植物（AUT）；以及(5) 未松弛、未创伤且未接种作为对照（CTR）。在接种后3至24小时内对超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和硫代巴比妥酸（TBARS）活性进行了定量分析。与未遭受氧化应激的CTR牡蛎相比，所有处理组的性腺和消化腺中SOD活性均显著降低，AUT牡蛎的外套膜组织中SOD活性也降低；这表明牡蛎珍珠养殖的整个准备过程（松弛、创伤和接种）会在宿主体内产生氧化应激。CAT并非用于测量牡蛎对创伤接种过程短期反应的敏感酶，而是对更持久或慢性应激较为敏感。与SOD类似，接种牡蛎中最低的GPx和TBARS活性证明了它们对氧化应激和损伤的易感性，尤其是在WOU处理组中。本研究证据表明，SOD是用于测量宿主牡蛎对赛博创伤和接种短期反应的更敏感酶。同样明显的是，宿主在珍珠养殖过程的所有阶段都会经历应激，主要是在性腺创伤期间，且与赛博的来源无关。
