基于壳聚糖和氧化羧甲基纤维素的水凝胶薄膜，经优化后可控制姜黄素释放，用于治疗皮肤病。

Hydrogel Films Based on Chitosan and Oxidized Carboxymethylcellulose Optimized for the Controlled Release of Curcumin with Applications in Treating Dermatological Conditions.

机构信息

Laboratory of Structure, Elaboration, and Application of Molecular Materials, Abdelhamid Ibn Badis University of Mostaganem, Mostaganem 27000, Algeria.

Faculty of Technology, Hassiba Benbouali University of Chlef, BP 151, Chlef 02000, Algeria.

出版信息

Molecules. 2021 Apr 10;26(8):2185. doi: 10.3390/molecules26082185.

Cross-linked chitosan (CS) films with aldehyde groups obtained by oxidation of carboxymethyl cellulose (CMC) with NaIO were prepared using different molar ratios between the CHO groups from oxidized carboxymethyl cellulose (CMCOx) and NH groups from CS (from 0.25:1 to 2:1). Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy demonstrated the aldehyde groups' presence in the CMCOx. The maximum oxidation degree was 22.9%. In the hydrogel, the amino groups' conversion index value increased when the -CHO/-NH molar ratio, cross-linking temperature, and time increased, while the swelling degree values decreased. The hydrogel films were characterized by scanning electron microscopy (SEM) and FTIR analysis. The curcumin encapsulation efficiency decreases from 56.74% to 16.88% when the cross-linking degree increases. The immobilized curcumin release efficiency (R%) and skin membrane permeability were evaluated in two different pH solutions using a Franz diffusion cell, and it was found to decrease when the molar ratio -CH=O/NH increases. The curcumin R% in the receptor compartment was higher at pH = 7.4 (18%- for the sample with a molar ratio of 0.25:1) than at pH = 5.5 (16.5%). The curcumin absorption in the skin membrane at pH = 5.5 (47%) was more intense than at pH = 7.4 (8.6%). The curcumin-loaded films' antioxidant activity was improved due to the CS presence.

用 NaIO 氧化羧甲基纤维素（CMC）得到醛基的交联壳聚糖（CS）薄膜，使用氧化羧甲基纤维素（CMCOx）中的 CHO 基团与 CS 中的 NH 基团的不同摩尔比（从 0.25:1 到 2:1）来制备。傅里叶变换红外（FTIR）和核磁共振（NMR）光谱表明 CMCOx 中存在醛基。最大氧化度为 22.9%。在水凝胶中，当 -CHO/-NH 摩尔比、交联温度和时间增加时，氨基转化率值增加，而溶胀度值降低。水凝胶膜通过扫描电子显微镜（SEM）和 FTIR 分析进行了表征。当交联度增加时，姜黄素的包封效率从 56.74%降低到 16.88%。使用 Franz 扩散池在两种不同的 pH 溶液中评估固定化姜黄素的释放效率（R%）和皮肤膜通透性，发现当 -CH=O/NH 摩尔比增加时，R%降低。在 pH = 7.4（对于摩尔比为 0.25:1 的样品，R%为 18%）时，受体腔内的姜黄素 R%高于 pH = 5.5（R%为 16.5%）。在 pH = 5.5（47%）时，姜黄素在皮肤膜中的吸收比在 pH = 7.4（8.6%）时更强烈。由于 CS 的存在，载有姜黄素的薄膜的抗氧化活性得到了提高。