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中国一株猪繁殖与呼吸综合征病毒重组株的遗传特征与致病性

Genetic Characterization and Pathogenicity of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Strain in China.

机构信息

National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

College of Agriculture, Hubei Three Gorges Polytechnic, Yichang 443000, China.

出版信息

Viruses. 2024 Jun 20;16(6):993. doi: 10.3390/v16060993.

DOI:10.3390/v16060993
PMID:38932283
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11209116/
Abstract

Since it was first reported in 2013, the NADC30-like PRRSV has been epidemic in China. Hubei Province is known as China's key hog-exporting region. To understand the prevalence and genetic variation of PRRSV, herein, we detected and analyzed 317 lung tissue samples from pigs with respiratory disease in Hubei Province, and demonstrated that the NADC30-like strain was the second-most predominant strain during 2017-2018, following the highly pathogenic PRRSV (HP-PRRSV). Additionally, we isolated a new NADC30-like PRRSV strain, named CHN-HB-2018, which could be stably passaged in Marc-145 cells. Genetic characterization analysis showed that compared with the NADC30 strain, the CHN-HB-2018 strain had several amino acid variations in glycoprotein (GP) 3, GP5, and nonstructural protein 2 (NSP2). Moreover, the CHN-HB-2018 strain showed a unique 5-amino acid (aa) deletion in NSP2, which has not previously been reported. Gene recombination analysis identified the CHN-HB-2018 strain as a potentially recombinant PRRSV of the NADC30-like strain and HP-PRRSV. Animal experiments indicated that the CHN-HB-2018 strain has a mild pathogenicity, with no mortality and only mild fever observed in piglets. This study contributes to defining the evolutionary characteristics of PRRSV and its molecular epidemiology in Hubei Province, and provides a potential candidate strain for PRRSV vaccine development.

摘要

自 2013 年首次报道以来，NADC30 样 PRRSV 在中国流行。湖北省是中国的主要生猪出口区。为了解 PRRSV 的流行情况和遗传变异，本研究检测和分析了湖北省 317 份来自患有呼吸道疾病的猪的肺组织样本，结果表明，在 2017-2018 年，NADC30 样毒株是仅次于高致病性 PRRSV（HP-PRRSV）的第二大主要流行株。此外，我们分离到一株新的 NADC30 样 PRRSV 株，命名为 CHN-HB-2018，该毒株能够在 Marc-145 细胞中稳定传代。遗传特征分析表明，与 NADC30 株相比，CHN-HB-2018 株在糖蛋白（GP）3、GP5 和非结构蛋白 2（NSP2）中具有几个氨基酸变异。此外，CHN-HB-2018 株在 NSP2 中具有一个独特的 5 个氨基酸（aa）缺失，这在以前尚未报道过。基因重组分析表明，CHN-HB-2018 株是 NADC30 样株和 HP-PRRSV 的潜在重组 PRRSV。动物实验表明，CHN-HB-2018 株具有轻度致病性，仔猪无死亡，仅观察到轻度发热。本研究有助于确定 PRRSV 的进化特征及其在湖北省的分子流行病学，并为 PRRSV 疫苗的开发提供了一个潜在的候选毒株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/6329115bfa06/viruses-16-00993-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/a4e28f091214/viruses-16-00993-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/6428e60e5ab8/viruses-16-00993-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/9b243717b55b/viruses-16-00993-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/6329115bfa06/viruses-16-00993-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/1e5d5d86971f/viruses-16-00993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/9f8e725ed957/viruses-16-00993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/2ac89de0fb4d/viruses-16-00993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/a4e28f091214/viruses-16-00993-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/6428e60e5ab8/viruses-16-00993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/ba4c003e7787/viruses-16-00993-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007c/11209116/6329115bfa06/viruses-16-00993-g008.jpg

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