Bahavarnia Farnaz, Bahari Hamed, Hasanzadeh Mohammad, Shadjou Nasrin
Nutrition Research Center, Tabriz University of Medical Sciences Tabriz Iran.
Food and Drug Safety Research Center, Tabriz University of Medical Sciences Tabriz Iran.
RSC Adv. 2024 Jun 26;14(28):20410-20419. doi: 10.1039/d4ra03575e. eCollection 2024 Jun 18.
Taurine is now widely used as a new biomarker for cardiovascular and neurodegenerative diseases. This study discusses the importance of accurately determining taurine biomarker levels in various tissues and fluids for the early diagnosis of important pathologies and diseases. Current methods for taurine analysis face challenges such as low sensitivity, lack of selectivity, and complex procedures. Therefore, an efficient analytical method/technique is urgently needed by clinicians. A new paper-based photochemical method using triangular silver nanoparticles (TA-AgNPs) as optical nanoprobes was developed to detect taurine in human blood plasma and urine samples. This method involves a chemical reaction between taurine and TA-AgNPs, leading to a color change at pH 4.8, which is detected using a paper-based colorimetry (PCD) assay. The reaction is further confirmed by UV-visible spectrophotometry as the interaction between taurine and TA-AgNPs causes a significant change in the absorption spectrum, enabling the rapid and reliable measurement of this important biomarker with a detection limit of less than 0.2 μM to 20 mM. The method has been successfully applied to bioanalyzing taurine in human body fluids. Additionally, it requires optimized single-drop paper/parafilm-based colorimetric devices (OD-PCDs) for and on-demand taurine analysis. This study represents the first use of TA-AgNPs for the specific and sensitive detection of taurine in real samples. The sensor design allows for the direct quantification of biomarkers in biological samples without the need for derivatization procedures or sample preparation. The simplicity and portability of OD-PCDs make them promising for tracking and monitoring. This method is expected to contribute to improving environmental health and occupational safety and represents a significant advancement in colorimetric analysis for the sensitive and selective detection of taurine, potentially providing a platform for the identification of taurine and other biomarkers.
牛磺酸现已被广泛用作心血管疾病和神经退行性疾病的新型生物标志物。本研究探讨了准确测定各种组织和体液中牛磺酸生物标志物水平对于重要病理和疾病早期诊断的重要性。当前用于牛磺酸分析的方法面临着诸如灵敏度低、缺乏选择性和程序复杂等挑战。因此，临床医生迫切需要一种高效的分析方法/技术。一种基于纸张的光化学新方法被开发出来，该方法使用三角形银纳米颗粒（TA-AgNPs）作为光学纳米探针来检测人血浆和尿液样本中的牛磺酸。该方法涉及牛磺酸与TA-AgNPs之间的化学反应，在pH 4.8时会导致颜色变化，通过基于纸张的比色法（PCD）测定来检测。通过紫外可见分光光度法进一步证实了该反应，因为牛磺酸与TA-AgNPs之间的相互作用会导致吸收光谱发生显著变化，从而能够快速可靠地测量这种重要的生物标志物，检测限低至0.2 μM至20 mM。该方法已成功应用于人体体液中牛磺酸的生物分析。此外，它需要优化的基于单滴纸/石蜡膜的比色装置（OD-PCD）用于按需进行牛磺酸分析。本研究首次使用TA-AgNPs对实际样品中的牛磺酸进行特异性和灵敏检测。该传感器设计允许直接定量生物样品中的生物标志物，而无需衍生化程序或样品制备。OD-PCD的简单性和便携性使其在跟踪和监测方面具有前景。该方法有望有助于改善环境卫生和职业安全，代表了比色分析在灵敏和选择性检测牛磺酸方面的重大进展，有可能为牛磺酸和其他生物标志物的鉴定提供一个平台。
