Laboratory Medicine Department, Hunan Prevention and Treatment Institute for Occupational Diseases, Changsha 410000, Hunan, China.
Department of Emergency, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, China.
J Food Drug Anal. 2024 Jun 15;32(2):239-249. doi: 10.38212/2224-6614.3503.
Cell nucleus status decides the activities of corresponding cells, making its rapid and effective staining important for revealing the actual condition of biological environment in life science and related fields. In this study, fast staining of cell nucleus is realized by fluorescent carbon nanodots (CDs). The staining mechanism is due to the positively charged CD surface-induced cell membrane penetration, which facilitates the CD-nucleus binding via electrostatic attraction. The size of cell nucleus is easily measured with fluorescence imaging technique. In addition, the CD-based cell nucleus stain is applied for discriminating the normal and cancer cells by determining the cell-to-nucleus ratio with fluorescence images.
细胞核状态决定相应细胞的活动，因此快速有效地对其进行染色对于揭示生命科学及相关领域中生物环境的实际情况非常重要。在本研究中，通过荧光碳纳米点（CDs）实现了细胞核的快速染色。这种染色机制归因于带正电荷的 CD 表面诱导细胞膜穿透，从而通过静电吸引促进 CD-细胞核的结合。通过荧光成像技术可以很容易地测量细胞核的大小。此外，通过荧光图像确定细胞与细胞核的比例，利用基于 CD 的细胞核染色来区分正常细胞和癌细胞。
