Micro-Nano System Research Centre & Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, China.
School of Energy and Power Engineering, North University of China, Taiyuan, 030051, China.
Anal Bioanal Chem. 2019 Feb;411(5):967-972. doi: 10.1007/s00216-018-1500-1. Epub 2019 Jan 2.
Carbon dots (Cdots) with bright green fluorescence were applied to the rapid and selective cell imaging for a variety of cell lines. Different labeling distributions of hepatoma cells (HepG2) and normal human liver cells (LO2) were achieved using Cdots as imaging agents. For HepG2 cells, the Cdots could rapidly permeate the cell membrane and diffuse into the cytoplasm and nucleus within 3 min, and retained their location in the targets for 24 h. However, the Cdots exhibited bright fluorescence only in the cytoplasm of LO2 cell lines. Moreover, the Cdots were almost non-cytotoxic and exhibited superior photostability over a wide range of pH. Therefore, these Cdots have great potential for rapid, luminous and selective bioimaging applications, and are expected to be used as a nucleus-staining agent in cancer diagnosis. Graphical abstract ᅟ.
具有明亮绿色荧光的碳点（Cdots）被应用于多种细胞系的快速和选择性细胞成像。使用 Cdots 作为成像剂，可以实现肝癌细胞（HepG2）和正常人类肝细胞（LO2）的不同标记分布。对于 HepG2 细胞，Cdots 可以在 3 分钟内快速穿透细胞膜，并扩散到细胞质和细胞核中，并且在 24 小时内保持在靶标位置。然而，Cdots 在 LO2 细胞系中仅显示出明亮的荧光。此外，Cdots 的细胞毒性几乎可以忽略不计，并且在很宽的 pH 范围内表现出优异的光稳定性。因此，这些 Cdots 具有快速、发光和选择性生物成像应用的巨大潜力，并有望用作癌症诊断中的核染色剂。
