Javed Fawad, Ahmed Hameeda Bashir, Saeed Anwar, Mehmood Abid, Bain Crawford
Imaging and Biomechanical Laboratory, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
J Periodontol. 2014 May;85(5):e130-5. doi: 10.1902/jop.2013.130514. Epub 2013 Oct 30.
The cytokine profile in unstimulated whole saliva (UWS) of patients with prediabetes and chronic periodontitis (CP) remains uninvestigated. The aim of this study is to assess interleukin (IL)-6 and matrix metalloproteinase (MMP)-8 levels in UWS of patients with CP with and without prediabetes.
Eighty-eight males (aged 39 to 51 years) were divided into three groups: group 1: 28 patients with CP and prediabetes; group 2: 30 patients with CP and without prediabetes; and group 3: 30 controls. Fasting blood glucose (FBG) and hemoglobin A1c (HbA1c) levels, periodontal parameters (plaque index, bleeding on probing, probing depth, attachment loss, and marginal bone loss), and number of missing teeth were recorded. UWS samples were collected, and UWS flow rate (UWSFR) was measured. IL-6 and MMP-8 were measured in UWS using enzyme-linked immunosorbent assay. P values <0.05 were considered statistically significant.
Mean FBG and HbA1c levels were significantly higher in group 1 (119.3 ± 3.1 mg/dL and 6.1% ± 0.2%) than group 2 (80.1 ± 3.5 mg/dL and 4.8% ± 0.5%; P <0.001) and group 3 (75.3 ± 2.2 mg/dL and 4.3% ± 0.2%; P <0.05). UWSFR was significantly higher in groups 2 (0.53 ± 0.1 mL/minute; P <0.05) and 3 (0.51 ± 0.1 mL/minute; P <0.01) than group 1 (0.33 ± 0.05 mL/minute). Periodontal parameters were worse in group 1 (P <0.05) and group 2 (P <0.05) than group 3. There was no difference in periodontal parameters, numbers of missing teeth, or salivary IL-6 and MMP-8 levels between patients in groups 1 and 2.
Salivary IL-6 and MMP-8 levels are elevated in patients with CP with and without prediabetes.
糖尿病前期患者和慢性牙周炎（CP）患者未刺激全唾液（UWS）中的细胞因子谱仍未得到研究。本研究的目的是评估患有和未患有糖尿病前期的CP患者UWS中白细胞介素（IL）-6和基质金属蛋白酶（MMP）-8的水平。
88名男性（年龄39至51岁）被分为三组：第1组：28例患有CP和糖尿病前期的患者；第2组：30例患有CP但无糖尿病前期的患者；第3组：30名对照者。记录空腹血糖（FBG）和糖化血红蛋白（HbA1c）水平、牙周参数（菌斑指数、探诊出血、探诊深度、附着丧失和边缘骨丧失）以及缺失牙数量。收集UWS样本并测量UWS流速（UWSFR）。使用酶联免疫吸附测定法测量UWS中的IL-6和MMP-8。P值<0.05被认为具有统计学意义。
第1组的平均FBG和HbA1c水平（119.3±3.1mg/dL和6.1%±0.2%）显著高于第2组（80.1±3.5mg/dL和4.8%±0.5%；P<0.001）和第3组（75.3±2.2mg/dL和4.3%±0.2%；P<0.05）。第2组（0.53±0.1mL/分钟；P<0.05）和第3组（0.51±0.1mL/分钟；P<0.01）的UWSFR显著高于第1组（0.33±0.05mL/分钟）。第1组（P<0.05）和第2组（P<0.05）的牙周参数比第3组更差。第1组和第2组患者之间的牙周参数、缺失牙数量或唾液IL-6和MMP-8水平没有差异。
患有和未患有糖尿病前期的CP患者唾液中IL-6和MMP-8水平均升高。
