Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Pimri, Pune, India.
In Vitro Cell Dev Biol Anim. 2024 Sep;60(8):926-934. doi: 10.1007/s11626-024-00937-9. Epub 2024 Jun 27.
Decellularized tissues are an attractive scaffolds for 3D tissue engineering. Decellularized animal tissues have certain limitations such as the availability of tissue, high costs and ethical concerns related to the use of animal sources. Plant-based tissue decellularized scaffolds could be a better option to overcome the problem. The leaves of different plants offer a unique opportunity for the development of tissue-specific scaffolds, depending on the reticulate or parallel veination. Herein, we decellularized spinach leaves and employed these for the propagation and osteogenic differentiation of dental pulp stem cells (DPSCs). DPSCs were characterized by using mesenchymal stem cell surface markers CD90, CD105 and CD73 and CD34, CD45 and HLA-DR using flow cytometry. Spinach leaves were decellularized using ethanol, NaOH and HCL. Cytotoxicity of spinach leaf scaffolds were analysed by MTT assay. Decellularized spinach leaves supported dental pulp stem cell adhesion, proliferation and osteogenic differentiation. Our data demonstrate that the decellularized spinach cellulose scaffolds can stimulate the growth, proliferation and osteogenic differentiation of DPSCs. In this study, we showed the versatile nature of decellularized plant leaves as a biological scaffold and their potential for bone regeneration in vitro.
去细胞化组织是 3D 组织工程的一种有吸引力的支架。去细胞化的动物组织存在一定的局限性，如组织的可用性、高成本以及与使用动物来源相关的伦理问题。植物来源的去细胞化组织支架可能是克服这些问题的更好选择。不同植物的叶子为开发组织特异性支架提供了独特的机会，这取决于网状脉或平行脉。在这里，我们对菠菜叶进行了去细胞化处理，并将其用于牙髓干细胞 (DPSC) 的增殖和成骨分化。通过流式细胞术，使用间充质干细胞表面标志物 CD90、CD105 和 CD73 以及 CD34、CD45 和 HLA-DR 对 DPSCs 进行了表征。使用乙醇、NaOH 和 HCL 对菠菜叶进行去细胞化处理。通过 MTT 分析检测菠菜叶支架的细胞毒性。去细胞化的菠菜叶支架支持牙髓干细胞的黏附、增殖和成骨分化。我们的数据表明，去细胞化的菠菜纤维素支架可以刺激 DPSCs 的生长、增殖和成骨分化。在这项研究中，我们展示了去细胞化植物叶子作为生物支架的多功能性及其在体外骨再生方面的潜力。
