Department of Life Science, Faculty of New Science and Technology, University of Tehran, Tehran, Iran.
Department of Life Science, Faculty of New Science and Technology, University of Tehran, Tehran, Iran.
Gene. 2020 Oct 5;757:144852. doi: 10.1016/j.gene.2020.144852. Epub 2020 Jun 27.
Until now, various methods have been introduced to fabricate 3D scaffolds to provide a suitable substrate for cell growth and proliferation and subsequent use in tissue engineering to repair damaged tissues. The 3D scaffolds can simulate the natural cellular microenvironment well. Herein, the decellularized leaf spinach has been used which not only have no problems associated with artificial scaffolds, but they also do not cost significantly. Decellularized scaffolds surface properties were characterized by the investigation of scaffolds surface roughness, hydrophilicity, mechanical properties, size and shape of porosities and specific surface area. In the next step, osteogenic differentiation potential of bone marrow derived mesenchymal stem cells cultured on the scaffold and culture plate (as a control) was evaluated using alizarin staining and calcium content, alkaline phosphatase activity and bone related genes expression assays. The results indicated that the surface properties and shape of scaffold pores were effective in the stem cells binding, growth and proliferation. This higher biocompatibility due to the ideal surface hydrophilicity as well as high specific surface area due to the presence of a rough grid surface ultimately increased the efficiency of stem cell's bone differentiation. Taken together, it can be concluded that the decellularized spinach leaf scaffold, due to its easy availability, low prices and high efficiency, can be considered as a promising potential candidate for use as a proper substrate for stem cell growth and differentiation in bone tissue engineering.
迄今为止，已经引入了各种方法来制造 3D 支架，为细胞生长和增殖提供合适的基质，并随后用于组织工程修复受损组织。3D 支架可以很好地模拟天然细胞微环境。在此，使用了脱细胞的菠菜叶，它不仅没有与人工支架相关的问题，而且成本也不高。通过研究支架表面粗糙度、亲水性、机械性能、孔隙率大小和形状以及比表面积来表征脱细胞支架的表面性质。在下一步中，通过茜素红染色和钙含量、碱性磷酸酶活性和骨相关基因表达测定来评估骨髓间充质干细胞在支架和培养板（作为对照）上的成骨分化潜力。结果表明，支架孔的表面性质和形状有利于干细胞的黏附、生长和增殖。由于理想的表面亲水性和粗糙网格表面的高比表面积，导致更高的生物相容性，最终提高了干细胞的成骨分化效率。综上所述，由于脱细胞菠菜叶支架易于获得、价格低廉、效率高，可以考虑将其作为干细胞生长和分化的合适基质，用于骨组织工程。
